Methods and apparatus for improved luminescence assays using a soluble polymer having a ligand and a plurality of electrochemiluminescent labels
申请人:Meso Scale Technologies, LLC
公开号:EP2284277A1
公开(公告)日:2011-02-16
The invention provides a reagent for carrying out ECL assays for an analyte-of-interest comprising an assay-ligand, said assay-ligand being linked to a soluble polymer comprising a plurality of electrochemiluminescence moieties.
Also provided is a complex comprising: (a) an analyte-of-interest; (b) an assay-ligand linked to a soluble polymer, said polymer comprising a plurality of electrochemiluminescent moieties; and (c) an assay ligand immobilized on an electrode.
Further provided is a method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of: (a) forming a complex comprising (i) an assay-ligand linked to a soluble polymer, said polymer comprising a plurality of ECL moieties, said assay-ligand being capable of binding with said analyte or with; (ii) an assay-ligand immobilized on an electrode; and (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
本发明提供了一种用于对感兴趣的分析物进行 ECL 检测的试剂,该试剂包括一种检测配体,所述检测配体与一种可溶性聚合物相连,所述聚合物包括多个电化学发光分子。
还提供了一种复合物,包括:(a) 所关注的分析物;(b) 与可溶性聚合物连接的检测配体,所述聚合物包括多个电致化学发光分子;以及 (c) 固定在电极上的检测配体。
此外,还提供了一种对感兴趣的结合分析物进行电化学发光测量的方法,包括以下步骤(a) 形成复合物,该复合物包括:(i) 与可溶性聚合物连接的检测配体,所述聚合物包括多个 ECL 分子,所述检测配体能够与所述分析物或与之结合;(ii) 固定在电极上的检测配体;以及 (b) 在存在电化学发光反应物的情况下,在所述电极上进行电化学发光测量。