MQAE(氯离子荧光探针) | 162558-52-3

• 物质功能分类: 生物 - 信号转导
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: MQAE(氯离子荧光探针)
• 中文别名: 解毒喹
• 英文名称: 1-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide
• 英文别名: ethyl 2-(6-methoxyquinolin-1-ium-1-yl)acetate;bromide
• CAS: 162558-52-3
• 化学式: C14H16BrNO3
• 分子量: 326.19
• InChiKey: DSLLHVISNOIYHR-UHFFFAOYSA-M

物化性质

• 熔点：
  177-179 °C(lit.)
• 溶解度：
  可溶于DMSO
• 最大波长(λmax)：
  350 nm

计算性质

• 辛醇/水分配系数(LogP)：
  -1.3
• 重原子数：
  19
• 可旋转键数：
  5
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.29
• 拓扑面积：
  39.4
• 氢给体数：
  0
• 氢受体数：
  4

安全信息

• 危险品标志：
  Xi
• 海关编码：
  2933499090
• 危险性防范说明：
  P261,P305+P351+P338
• 危险性描述：
  H315,H319,H335

制备方法与用途

生物活性

MQAE 是一种荧光氯离子指示剂，其灵敏度和选择性均优于 36Cl 同位素示踪法及微电极法。

体外研究

将 MQAE 应用于急性脑切片时，能高质量地标记神经细胞及其过程。MQAE 的荧光信号足够强大且与测量单个细胞的 cAMP 依赖性氯离子运输相当。此外，MQAE 还可用于测定大鼠主动脉平滑肌细胞（VSMC）内的氯离子浓度。

文献信息

• Tetrahydroisoquinoline derivatives for treating protein trafficking diseases
  申请人：Pregel J. Marko

  公开号：US20050176761A1

  公开（公告）日：2005-08-11

  Tetrhydroisoquinoline derivatives, pharmaceutical compositions comprising them and methods of treating disease are disclosed herein. The disclosed compounds are useful in the treatment and prevention of diseases mediated by chloride channel activity and/or protein trafficking, including, but not limited to, diseases associated with impaired mucociliary clearance such as cystic fibrosis, bronchitis, emphysema, and the like.

  本文披露了四氢异喹啉衍生物、包含它们的药物组合物以及治疗疾病的方法。所披露的化合物在治疗和预防由氯离子通道活性和/或蛋白质运输介导的疾病方面是有用的，包括但不限于与黏液纤毛清除受损有关的疾病，如囊性纤维化、支气管炎、肺气肿等。
• p16 mediated regulation of NMDA receptors
  申请人：Nakanishi Nobuki

  公开号：US20050037433A1

  公开（公告）日：2005-02-17

  Discovered is a novel protein and variants thereof whose activity at the NMDA receptor causes an increased efflux of calcium ions through the channel of said receptor. This activity is downregulated by the NR3A subunit of NMDA. Also discovered are the nucleic acid sequences encoding said novel protein and variants thereof. The discovery is useful for the diagnosing of NMDA receptor dysregulation and the treatment of NMDA receptor dysregulation related disorders. In addition, the discovery is useful for the further discovery of modulators affecting the activity of the novel protein and variants thereof at the NMDA receptor.

  发现了一种新的蛋白质及其变异体，其在NMDA受体上的活性通过该受体通道导致钙离子外流增加。该活性被NMDA的NR3A亚单位下调。还发现了编码该新蛋白质及其变异体的核酸序列。该发现对于诊断NMDA受体失调和治疗相关疾病非常有用。此外，该发现对于进一步发现影响新蛋白质及其变异体在NMDA受体上的活性的调节剂也非常有用。
• p16 MEDIATED REGULATION OF NMDA RECEPTORS
  申请人：Nakanishi Nobuki

  公开号：US20120046446A1

  公开（公告）日：2012-02-23

  Discovered is a novel protein and variants thereof whose activity at the NMDA receptor causes an increased efflux of calcium ions through the channel of said receptor. This activity is downregulated by the NR3A subunit of NMDA. Also discovered are the nucleic acid sequences encoding said novel protein and variants thereof. The discovery is useful for the diagnosing of NMDA receptor dysregulation and the treatment of NMDA receptor dysregulation related disorders. In addition, the discovery is useful for the further discovery of modulators affecting the activity of the novel protein and variants thereof at the NMDA receptor.

  发现了一种新型蛋白质及其变异体，其在NMDA受体上的活性通过该受体通道引起钙离子的增加流出。该活性受到NMDA的NR3A亚基的下调调节。还发现了编码该新型蛋白质及其变异体的核酸序列。该发现对于诊断NMDA受体失调以及治疗与NMDA受体失调相关的疾病非常有用。此外，该发现对于进一步发现影响新型蛋白质及其变异体在NMDA受体上的活性的调节剂也非常有用。
• METHOD OF MEASUREMENT OF MATERIAL AND TESTPIECE
  申请人：KABUSHIKI KAISHA KYOTO DAIICHI KAGAKU

  公开号：EP0860695A1

  公开（公告）日：1998-08-26

  A method of measuring an analyte, comprising a step of measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein a layered inorganic compound is caused to exist in the reaction system including the formation reaction of the detectable substance, whereby high-sensitivity measurement is made possible, the detectable substance can be stabilized to improve accuracy of the measurement, a rate of a chemical reaction is increased to enable quick measurement, and high-sensitivity measurement is made possible even in a reaction system which forms an insoluble substance. Also, it can be provided an analytical testing piece for measuring an analyte, by measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein the testing piece comprises at least one test portion having a detection portion for detecting the detectable substance and contains a layered inorganic compound at least in the test portion, whereby diffusion and elution of a dyestuff or the like is prevented, more sensitive and accurate simple analysis is made possible, and easy handling is possible.

  一种测量被分析物的方法，包括以下步骤：根据样品中含有的被分析物的化学反应，使用包括可检测物质的形成反应的反应系统测量可检测物质，其中在包括可检测物质的形成反应的反应系统中导致层状无机化合物的存在、从而实现高灵敏度测量，稳定可检测物质以提高测量精度，提高化学反应速率以实现快速测量，即使在形成不溶性物质的反应体系中也能实现高灵敏度测量。此外，还可以提供一种用于测量被分析物的分析检测件，通过使用反应系统测量可检测物质，该反应系统包括基于样品中所含被分析物的化学反应的可检测物质的形成反应，其中检测件包括至少一个测试部分，该测试部分具有用于检测可检测物质的检测部分，并且至少在测试部分中包含分层无机化合物，从而防止染料或类似物的扩散和洗脱，实现更灵敏、更准确的简单分析，并且易于处理。
• SPECIMEN HAVING CAPABILITY OF SEPARATING SOLID COMPONENT
  申请人：ARKRAY, Inc.

  公开号：EP1271143A1

  公开（公告）日：2003-01-02

  A test strip having the capability of separating blood cells from whole blood, wherein it has a porous reagent layer comprising beads, an inorganic gel and a reagent that causes a detectable reaction with a substance to be detected, and a substrate that supports the reagent layer, wherein the beads are adhered to each other with the inorganic gel, and interstices are formed between the beads to trap a solid. The test strip can thus be used for separating blood cells from a whole blood including blood cells and plasma and detecting a substance to be detected, such as glucose, contained in plasma. The use of the test strip allows the measurement of a substance to be detected even by measurement of a transmitted light, the exhibition of good oxygen permeability, and thus can be used for measuring a substance to be detected with improved accuracy.

  一种能从全血中分离血细胞的试纸，它具有一个多孔试剂层，该试剂层由微珠、无机凝胶和能与待检测物质发生可检测反应的试剂组成，以及一个支撑试剂层的基板，其中微珠与无机凝胶相互粘附，微珠之间形成间隙以吸附固体。因此，该试纸可用于从包括血细胞和血浆在内的全血中分离出血细胞，并检测血浆中的待检测物质，如葡萄糖。使用该试纸甚至可以通过透射光的测量来检测某种物质，显示出良好的透氧性，从而可以更准确地测量某种待检测物质。