2-Nitrophenyl-beta-D-galactopyranoside | 666699-73-6

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: 2-Nitrophenyl-beta-D-galactopyranoside
• 英文别名: o-nitrophenyl b-D-galactopyranoside；ONPG；2-(Hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
• CAS: 666699-73-6
• 化学式: C₁₂H₁₅NO₈
• mdl: MFCD00370360
• 分子量: 301.253
• InChiKey: KUWPCJHYPSUOFW-UHFFFAOYSA-N

物化性质

• 沸点：
  572.4±50.0 °C(Predicted)
• 密度：
  1.599±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -1.2
• 重原子数：
  21
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  145
• 氢给体数：
  4
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  2-Nitrophenyl-beta-D-galactopyranoside 、 disodium;carbonate 反应 1.0h， 生成 硝苯酚
  参考文献：
  名称：

  Sterol regulatory element binding proteins and their use in screening

  摘要：

  本发明揭示了一种核蛋白，该核蛋白结合甾体调节元件（SRE），例如低密度脂蛋白（LDL）受体基因的SRE-1，并介导LDL受体基因的甾体调节转录。此外，还描述了筛选测定和用于识别促进LDL受体基因转录的药剂的方法，以用于降低血浆胆固醇和治疗相关的各种医学问题。

  公开号：

  US05498696A1
• 作为产物：
  描述：

  Sodium phosphate, tribasic- 、 尾-D-Galactoside,2-nitrophenyl(9CI) 、 碳酸氢钠 、 在 ice 、 polyacrylamide 作用下， 以 甲苯 为溶剂， 生成 2-Nitrophenyl-beta-D-galactopyranoside
  参考文献：
  名称：

  Thermal stable beta-galactosidase

  摘要：

  一种热稳定的β-半乳糖苷酶酶来自新的Bacillus stearothermophilus菌株。该β-半乳糖苷酶适用于在至少55℃的温度下水解乳糖，特别适用于在至少65℃的温度下使用。对于乳糖的水解，β-半乳糖苷酶酶可以是经过大量纯化的酶或含有β-半乳糖苷酶酶的固定化整个细胞的形式。

  公开号：

  US04332895A1

文献信息

• Cleavable conjugates of antibiotics and an antibacterial cell-penetrating peptide
  申请人：Purdue Research Foundation

  公开号：US20190038759A1

  公开（公告）日：2019-02-07

  The present disclosure relates to novel cleavable conjugates of antibiotics and an antibacterial cell-penetrating peptide, and methods to make and use the novel cleavable conjugates of antibiotics and an antibacterial cell-penetrating peptide.

  本公开涉及新型可断裂的抗生素和抗菌细胞穿透肽的结合物，以及制备和使用这种新型可断裂的抗生素和抗菌细胞穿透肽的结合物的方法。
• B. anthracis prevention and treatment: mutant B. anthracis lacking luxS activity and furanone inhibition of growth, AI-2 quorum sensing, and toxin production
  申请人：Jones B. Marcus

  公开号：US20050042634A1

  公开（公告）日：2005-02-24

  The present invention pertains to the discovery that B. anthracis possesses a luxS gene that encodes a functional LuxS polypeptide, and that B. anthracis synthesizes a functional AI-2 quorum-sensing molecule. The invention provides mutant B. anthracis bacteria lacking the function of the luxS gene, which do not produce a functional AI-2 molecule and have growth defects compared to wild-type B. anthracis . The invention also concerns methods for inhibiting the growth of B. anthracis , or for preventing or treating B. anthracis infection, by inhibiting the activity of the B. anthracis LuxS polypeptide, or by exposure of the B. anthracis to furanone. In particular, the invention concerns the use of furanone, a compound that inhibits AI-2-mediated quorum-sensing, to inhibit the growth of B. anthracis , to inhibit B. anthracis toxin production, particularly that of protective antigen, and to prevent or treat B. anthracis infection. The invention also provides methods to prevent B. anthracis infection, or enhance an immune response to B. anthracis infection, by administering a vaccine comprising a B. anthracis cell in which the luxS gene is mutated.

  本发明涉及发现B. anthracis具有编码功能性LuxS多肽的luxS基因，以及B. anthracis合成功能性AI-2类群体感应分子。该发明提供了缺乏luxS基因功能、不产生功能性AI-2分子并与野生型B. anthracis相比具有生长缺陷的突变B. anthracis细菌。本发明还涉及通过抑制B. anthracis LuxS多肽的活性或暴露于呋喃酮来抑制B. anthracis的生长，预防或治疗B. anthracis感染的方法。特别是，本发明涉及使用呋喃酮，一种抑制AI-2介导的类群体感应的化合物，来抑制B. anthracis的生长，抑制B. anthracis毒素的产生，特别是保护性抗原的产生，并预防或治疗B. anthracis感染。本发明还提供了通过给予包含luxS基因突变的B. anthracis细胞的疫苗来预防B. anthracis感染或增强对B. anthracis感染的免疫反应的方法。
• Thermostable strains of the genus thermus capable of producing a .beta.
  申请人：Research Development Corporation of Japan

  公开号：US05432078A1

  公开（公告）日：1995-07-11

  Biologically pure strains of Thermus sp. FERM BP-1678, FERM BP-1679 and FERM BP-1680 have been isolated, and are capable of producing a beta-galactosidase. The beta-galactosidase produced by these strains has specific characteristics wherein the enzyme is optimum at a temperature of 75 to 85 degrees celsius and pH of 4.5 to 6.5. Further, the enzymatic activity is reduced by at most 10% in the presence of 50 millimoles (mM) of galactose and glucose, respectively.

  翻译结果：已经分离出生物学纯净的Thermus sp. FERM BP-1678、FERM BP-1679和FERM BP-1680菌株，并且具有产生β-半乳糖苷酶的能力。这些菌株产生的β-半乳糖苷酶具有特定的特性，其中酶在温度为75至85摄氏度和pH为4.5至6.5时最适宜。此外，在存在50毫摩尔（mM）的半乳糖和葡萄糖时，酶活性最多会降低10%。
• Sterol regulatory element binding proteins and their use in screening
  申请人：Board of Regents, The University of Texas System

  公开号：US05498696A1

  公开（公告）日：1996-03-12

  A nuclear protein which binds sterol regulatory elements (SREs), such as SRE-1 of the low density lipoprotein (LDL) receptor gene, and mediates sterol-regulated transcription of the LDL receptor gene is disclosed. Also described are screening assay and methods for the identification of agents capable of promoting LDL receptor gene transcription for use in reducing plasma cholesterol and treating the various medical problems associated therewith.

  本发明揭示了一种核蛋白，该核蛋白结合甾体调节元件（SRE），例如低密度脂蛋白（LDL）受体基因的SRE-1，并介导LDL受体基因的甾体调节转录。此外，还描述了筛选测定和用于识别促进LDL受体基因转录的药剂的方法，以用于降低血浆胆固醇和治疗相关的各种医学问题。
• Process for controlling cultures of recombinants
  申请人：Hitachi, Ltd.

  公开号：US05674678A1

  公开（公告）日：1997-10-07

  Expression of a desired gene and production of a product from the desired gene can be obtained effectively by cultivating a microorganism containing a hybrid plasmid comprising the desired gene, a vector and a promoter in its cell, and adding an inducer and a nutriment simultaneously to the culture medium at the time when the nutriment originally present in the culture medium is almost consumed.

  通过培养含有所需基因、载体和启动子的杂交质粒的微生物，并在营养物质几乎被消耗完时同时向培养基中添加诱导剂和营养物质，可以有效地表达所需基因并生产所需基因的产物。