2-Cyano-3-(3,4-dihydroxyphenyl)-N-(benzyl)-2-propenamide

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 肉桂酸及其衍生物
• 英文名称: 2-Cyano-3-(3,4-dihydroxyphenyl)-N-(benzyl)-2-propenamide
• 英文别名: (Z)-N-benzyl-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enamide
• 化学式: C₁₇H₁₄N₂O₃
• 分子量: 294.3
• InChiKey: TUCIOBMMDDOEMM-ZSOIEALJSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.4
• 重原子数：
  22
• 可旋转键数：
  4
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.06
• 拓扑面积：
  93.4
• 氢给体数：
  3
• 氢受体数：
  4

文献信息

• COMPOSITION FOR TRANSFERRING GENE TO CELL
  申请人：Ishihara Sangyo Kaisha, Ltd.

  公开号：EP3020809A1

  公开（公告）日：2016-05-18

  Disclosed are a composition for gene transfer, a method for introducing a gene into cell of a non-human animal, a method for introducing a gene into an in vitro cell, and a kit for gene transfer. The composition comprises (A) at least one lipid selected from the group consisting of sorbitan sesquioleate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan trioleate, sorbitan monooleate, L-α-phosphatidylinositol, L-α-dioleoyl phosphatidylethanolamine, octadecylamine, hexadecylamine, DOTAP, and cardiolipin; (B) at least one protein selected from the group consisting of albumin, casein, gelatin, and sericin; and (C) at least one positively charged substance selected from the group consisting of protamine sulfate, polyarginine, polylysine, polyethyleneimine, and hexadimethrine bromide.

  本发明公开了一种基因转移组合物、一种将基因导入非人类动物细胞的方法、一种将基因导入体外细胞的方法和一种基因转移试剂盒。组合物包括 (A) 至少一种选自山梨糖醇倍半油酸酯、山梨糖醇单月桂酸酯、山梨糖醇单棕榈酸酯、山梨糖醇单硬脂酸酯、山梨糖醇三油酸酯、山梨糖醇单油酸酯、L-α-磷脂酰肌醇、L-α-二油酰磷脂酰乙醇胺、十八胺、十六胺、DOTAP 和心磷脂的脂质；(B) 至少一种蛋白质，选自由白蛋白、酪蛋白、明胶和丝胶蛋白组成的组；以及 (C) 至少一种带正电荷的物质，选自由硫酸原胺、聚精氨酸、聚赖氨酸、聚乙烯亚胺和溴化六甲亚胺组成的组。
• COMPOSITIONS AND METHODS FOR IDENTIFYING LATENTLY INFECTED CELLS
  申请人：THE J. DAVID GLADSTONE INSTITUTES

  公开号：US20160186210A1

  公开（公告）日：2016-06-30

  The present disclosure provides for recombinant nucleic acids, and cells and virions comprising the recombinant nucleic acids, that can be used to identify, isolate, and/or purify cells latently infected with immunodeficiency virus. A subject recombinant nucleic acid includes (a) a first nucleotide sequence encoding a first reporter polypeptide that produces a first detectable signal, where the first nucleotide sequence is operably linked to an immunodeficiency virus promoter and is translated as an early gene; and (b) a second nucleotide sequence encoding a second reporter polypeptide that produces a second detectable signal that is distinguishable from the first detectable signal, where the second nucleotide sequence is operably linked to a non-immunodeficiency virus promoter. In some aspects, the first and second nucleotide sequences are both positioned between a shared 5′ long terminal repeat (LTR) and a shared 3′ LTR. Also provided are related methods.