3,3-dimethylpentanedioic acid (6-bromo-4-diazomethyl-2-oxo-2H-chromen)-7-yl 2-[4-(6-bromo-4-diazomethyl-2-oxo-2H-chromen-7-yloxycarbonyl)-3,3-dimethylbutyryloxy]ethyl ester | 909108-30-1

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 3,3-dimethylpentanedioic acid (6-bromo-4-diazomethyl-2-oxo-2H-chromen)-7-yl 2-[4-(6-bromo-4-diazomethyl-2-oxo-2H-chromen-7-yloxycarbonyl)-3,3-dimethylbutyryloxy]ethyl ester
• 英文别名: 1-O-[6-bromo-4-(diazomethyl)-2-oxochromen-7-yl] 5-O-[2-[5-[6-bromo-4-(diazomethyl)-2-oxochromen-7-yl]oxy-3,3-dimethyl-5-oxopentanoyl]oxyethyl] 3,3-dimethylpentanedioate
• CAS: 909108-30-1
• 化学式: C₃₆H₃₂Br₂N₄O₁₂
• 分子量: 872.478
• InChiKey: UWPUZDHPNGXAFB-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  5.5
• 重原子数：
  54
• 可旋转键数：
  19
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.33
• 拓扑面积：
  162
• 氢给体数：
  0
• 氢受体数：
  14

反应信息

• 作为产物：
  描述：

  3,3-二甲基戊二酸酐 在 氯化亚砜 、 三乙胺 作用下， 以 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 反应 52.0h， 生成 3,3-dimethylpentanedioic acid (6-bromo-4-diazomethyl-2-oxo-2H-chromen)-7-yl 2-[4-(6-bromo-4-diazomethyl-2-oxo-2H-chromen-7-yloxycarbonyl)-3,3-dimethylbutyryloxy]ethyl ester
  参考文献：
  名称：

  Crosslinking agent, crosslinking method, method of controlling gene expression, and method of examining gene function

  摘要：

  本发明提供了一种交联剂，其在两端具有光降解保护基团，用于交联双链核酸、核酸和蛋白质或多肽，或蛋白质或多肽，特别是双链RNA；一种使用该交联剂交联双链RNA或类似物的方法；一种调节基因表达的方法，可以在任意时机和位置控制目标基因的表达；以及一种检查基因功能的方法。根据本发明，双链核酸之间、核酸和蛋白质或多肽之间，或蛋白质或多肽之间，特别是双链RNA之间的交联可以很容易地形成，此外，交联也可以很容易地去除，因此可以高效地在任意时机和位置轻松控制目标基因的表达。因此，可以执行在特定时机和位置表达的基因的功能检查和/或鉴定。此外，可以抑制传统光遮蔽化合物难以抑制的双链RNA（siRNA）的RNAi效应，并且可以在任意时机和位置轻松控制目标基因的表达。

  公开号：

  US08084625B2

文献信息

• CROSSLINKING AGENT, CROSSLINKING METHOD, METHOD OF CONTROLLING GENE EXPRESSION, AND METHOD OF EXAMINING GENE FUNCTION
  申请人：Furuta Toshiaki

  公开号：US20090023140A1

  公开（公告）日：2009-01-22

  The present invention provides a crosslinking agent which have photodegradable protective groups at two ends to crosslink double-stranded nucleic acid, a nucleic acid and a protein or a polypeptide, or proteins or polypeptides, in particular, double-stranded RNA; a method for crosslinking a double-stranded RNA or the like using the same; a method for regulating gene expression, which can control the expression of a target gene at an arbitrary timing and location; and a method for examining a gene function. According to the present invention, crosslinking between double-stranded nucleic acids between a nucleic acid and a protein or a polypeptide, or between proteins or polypeptides, in particular, between double-stranded RNA can be easily formed, and in addition, the crosslinking can also be easily removed, so that the expression of a target gene can be easily controlled at an arbitrary timing and location with high efficiency. Hence, as a result, function examination and/or identification of a gene that is expressed at a specific timing and location can be performed. In addition, the RNAi effect of a double-stranded RNA (siRNA) that cannot be easily inhibited by a conventional caged compound can be inhibited, and the expression of a target gene can be easily controlled at an arbitrary timing and location.
• US8084625B2
  申请人：——

  公开号：US8084625B2

  公开（公告）日：2011-12-27
• Crosslinking agent, crosslinking method, method of controlling gene expression, and method of examining gene function
  申请人：Wako Pure Chemical Industries, Ltd.

  公开号：US08084625B2

  公开（公告）日：2011-12-27

  The present invention provides a crosslinking agent which have photodegradable protective groups at two ends to crosslink double-stranded nucleic acid, a nucleic acid and a protein or a polypeptide, or proteins or polypeptides, in particular, double-stranded RNA; a method for crosslinking a double-stranded RNA or the like using the same; a method for regulating gene expression, which can control the expression of a target gene at an arbitrary timing and location; and a method for examining a gene function. According to the present invention, crosslinking between double-stranded nucleic acids between a nucleic acid and a protein or a polypeptide, or between proteins or polypeptides, in particular, between double-stranded RNA can be easily formed, and in addition, the crosslinking can also be easily removed, so that the expression of a target gene can be easily controlled at an arbitrary timing and location with high efficiency. Hence, as a result, function examination and/or identification of a gene that is expressed at a specific timing and location can be performed. In addition, the RNAi effect of a double-stranded RNA (siRNA) that cannot be easily inhibited by a conventional caged compound can be inhibited, and the expression of a target gene can be easily controlled at an arbitrary timing and location.

  本发明提供了一种交联剂，其在两端具有光降解保护基团，用于交联双链核酸、核酸和蛋白质或多肽，或蛋白质或多肽，特别是双链RNA；一种使用该交联剂交联双链RNA或类似物的方法；一种调节基因表达的方法，可以在任意时机和位置控制目标基因的表达；以及一种检查基因功能的方法。根据本发明，双链核酸之间、核酸和蛋白质或多肽之间，或蛋白质或多肽之间，特别是双链RNA之间的交联可以很容易地形成，此外，交联也可以很容易地去除，因此可以高效地在任意时机和位置轻松控制目标基因的表达。因此，可以执行在特定时机和位置表达的基因的功能检查和/或鉴定。此外，可以抑制传统光遮蔽化合物难以抑制的双链RNA（siRNA）的RNAi效应，并且可以在任意时机和位置轻松控制目标基因的表达。