Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.
本发明公开了从
纤维素生物质样品中提取和定量
纤维素的解决方案和方法。通过使用萃取溶剂混合物来提高萃取率和选择性,该混合物包括非烷基溶剂(如
DMSO)、季
铵盐(如 TBAF)和季
铵碱(如 TBAOH)。提取的
纤维素可以选择使用已公开的沉淀溶液进行沉淀,以进一步提高
纤维素的纯度。提取的
纤维素可通过将
纤维素水解为
葡萄糖或纤维
生物糖,或使用已公开的
纤维素-盐复合物分光光度法进行测定。