[2-(4-乙酰氧基苯基)乙酰基] 2-(4-乙酰氧基苯基)乙酸酯 | 79524-46-2

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: [2-(4-乙酰氧基苯基)乙酰基] 2-(4-乙酰氧基苯基)乙酸酯
• 中文别名: [2-(4-乙酰氧基苯基)乙酰基]2-(4-乙酰氧基苯基)乙酸酯
• 英文名称: p-Acetoxyphenylacetic anhydride
• 英文别名: [4-(Acetyloxy)phenyl]acetic anhydride；[2-(4-acetyloxyphenyl)acetyl] 2-(4-acetyloxyphenyl)acetate
• CAS: 79524-46-2
• 化学式: C₂₀H₁₈O₇
• 分子量: 370.359
• InChiKey: CFDGBXLHNPXKJT-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.6
• 重原子数：
  27
• 可旋转键数：
  10
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.2
• 拓扑面积：
  96
• 氢给体数：
  0
• 氢受体数：
  7

反应信息

• 作为反应物：
  描述：

  [2-(4-乙酰氧基苯基)乙酰基] 2-(4-乙酰氧基苯基)乙酸酯 在 吡啶 、 碳酸氢钠 作用下， 以 甲醇 为溶剂， 反应 36.0h， 生成 19-(p-Hydroxyphenylacetoxy)-4-androstene-3,17-dione
  参考文献：
  名称：

  A chemical approach to solving bridging phenomena in steroid radioimmunoassays

  摘要：

  Steroid radioimmunoassays (RIA) employ antibodies raised against a carrier protein-steroid conjugate. Individual antibodies may recognize the steroid, the protein or the chemical bridge used to join them together. Use of the same bridge in the tracer results in higher affinity binding of the tracer than the native ligand which in turn results in a loss of sensitivity and precision. We have greatly reduced bridge-binding in a RIA for androstenedione. Conjugates and radioiodinated labels were prepared with either an ester or either chemical bridge. By using an antibody and the corresponding label with the heterologous bridge very sensitive assays were obtained.

  DOI：

  10.1016/0039-128x(81)90030-1
• 作为产物：
  描述：

  4-乙酰氧基苯乙酸 在 吡啶 、 氯化亚砜 作用下， 以 苯 为溶剂， 以73%的产率得到[2-(4-乙酰氧基苯基)乙酰基] 2-(4-乙酰氧基苯基)乙酸酯
  参考文献：
  名称：

  A chemical approach to solving bridging phenomena in steroid radioimmunoassays

  摘要：

  Steroid radioimmunoassays (RIA) employ antibodies raised against a carrier protein-steroid conjugate. Individual antibodies may recognize the steroid, the protein or the chemical bridge used to join them together. Use of the same bridge in the tracer results in higher affinity binding of the tracer than the native ligand which in turn results in a loss of sensitivity and precision. We have greatly reduced bridge-binding in a RIA for androstenedione. Conjugates and radioiodinated labels were prepared with either an ester or either chemical bridge. By using an antibody and the corresponding label with the heterologous bridge very sensitive assays were obtained.

  DOI：

  10.1016/0039-128x(81)90030-1

文献信息

• A specific radioimmunoassay for androstenedione with reduced bridge-binding
  作者：Gerald D. Nordblom、Raymond E. Counsell、Barry G. England

  DOI：10.1016/0039-128x(84)90009-6

  日期：1984.9

  Antibody used in a steroid radioimmunoassay raised against a steroid hapten-carrier protein conjugate may recognize both the hapten and the chemical bridge to the protein. Use of the same bridge in the radio-isotopic label may lead to higher affinity binding to the label than to the native steroid. Inhibition curves under these conditions are shallow and generally not acceptable for radioimmunoassay procedures. We have developed a radioimmunoassay for androstenedione that employs different bridges at the 11 beta position of the steroid for the protein conjugate and label. The resulting assay has greatly reduced bridge-binding, has an acceptable slope for the standard curve and is very specific as evidenced by low crossreactivies to other steroids.
• A chemical approach to solving bridging phenomena in steroid radioimmunoassays
  作者：G.D. Nordblom、R. Webb、R.E. Counsell、B.G. England

  DOI：10.1016/0039-128x(81)90030-1

  日期：1981.8

  Steroid radioimmunoassays (RIA) employ antibodies raised against a carrier protein-steroid conjugate. Individual antibodies may recognize the steroid, the protein or the chemical bridge used to join them together. Use of the same bridge in the tracer results in higher affinity binding of the tracer than the native ligand which in turn results in a loss of sensitivity and precision. We have greatly reduced bridge-binding in a RIA for androstenedione. Conjugates and radioiodinated labels were prepared with either an ester or either chemical bridge. By using an antibody and the corresponding label with the heterologous bridge very sensitive assays were obtained.