The regioselective and stereoselective hydroxylation of steroids by fungal strains previously known for their hydroxylation capabilities, such as Thamnostylum (= Helicostylum) piriforme ATCC 8992, Mucor griseocyanus ATCC 1207a, Actinomucor elegans (= Mucor parasiticus) MMP 3122 (Mucorales), and Zygodesmus sp. ATCC 14716, was investigated with special interest for the 14 alpha-hydroxylation reaction
以前以其羟基化能力而闻名的真菌菌株对类
固醇的区域选择性和立体选择性羟基化,例如 Thamnostylum (= Helicostylum) piriforme A
TCC 8992、Mucor griseocyanus A
TCC 1207a、Actinomucor elegans (= Mucor parasiticus) MMP 3122 (MMP. A
TCC 14716 对 14 α-羟基化反应特别感兴趣。初步筛选表明,其中一些微
生物足以产生以下类
固醇的 14 种 α-羟基化衍
生物:
孕酮、5 β-孕烷-3,20-二酮、3 β-羟基-5 β-孕烷-20 -one、3 beta-hydroxy-5 beta-17 (alpha H)-etianic酸甲酯、androst-4-ene-3,17-dione 和
睾酮。大约 20 种代谢物已通过
硅胶色谱法和半制备型反相 HPLC 分离和纯化。这些代谢物已通过