A two-photon probe for real-time monitoring of intracellular magnesium ions is provided. Specifically, the two-photon probe is represented by Formula 1:
wherein R is H or CH2OCOCH3.
The two-photon probe is very suitable for real-time imaging of intracellular magnesium ions. The two-photon probe shows 17-fold two-photon excited fluorescence enhancement in response to Mg2+, which is 7-fold stronger than commercial probes, thus enabling staining of cells in a greatly reduced amount. In addition, the two-photon probe has a sufficiently low molecular weight to stain cells and is very suitable for monitoring Mg2+ ions present in the deep tissue. Furthermore, the two-photon probe can be effectively used for the quantitative as well as qualitative detection of intracellular magnesium ions. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular magnesium ions using the two-photon probe.
提供一个用于实时监测细胞内
镁离子的双光子探针。具体而言,所提供的双光子探针由公式1表示:其中R是H或CH2OCOCH3。该双光子探针非常适合用于实时成像细胞内
镁离子。该双光子探针对Mg2+的反应显示出17倍的双光子激发荧光增强,比商业探针强7倍,因此能够在极大地减少的量中对细胞进行染色。另外,该双光子探针具有足够低的分子量以对细胞进行染色,并且非常适合监测存在于深层组织中的Mg2+离子。此外,该双光子探针可以有效地用于定性和定量检测细胞内
镁离子。进一步提供了一种制备双光子探针的方法以及一种使用双光子探针实时监测细胞内
镁离子的方法。