6-[[(3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]methylamino]-1H-pyrimidin-2-one

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: 6-[[(3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]methylamino]-1H-pyrimidin-2-one
• 化学式: C₁₁H₁₇N₃O₆
• 分子量: 287.27
• InChiKey: HIGGUKJTPRKCAA-BKEDMCBOSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -2.7
• 重原子数：
  20
• 可旋转键数：
  4
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.64
• 拓扑面积：
  144
• 氢给体数：
  6
• 氢受体数：
  7

文献信息

• Compositions and methods for analyzing modified nucleotides
  申请人：New England Biolabs, Inc.

  公开号：US10619200B2

  公开（公告）日：2020-04-14

  A method for identifying the location and phasing of modified cytosines (C) in long stretches of nucleic acids is provided. In some embodiments, the method may comprise (a) reacting a first portion of a nucleic acid sample containing at least one C and/or at least one modified C with a DNA glucosyltransferase and a cytidine deaminase to produce a first product and optionally reacting a second portion of the sample with a dioxygenase and a cytidine deaminase to produce a second product and; (b) comparing the sequences from the first and optionally the second product obtained in (a), or amplification products thereof, with each other and/or an untreated reference sequence to determine which Cs in the initial nucleic acid fragment are modified. A modified TET methylcytosine dioxygenase that is more efficient at converting methylcytosine to carboxymethylcytosine is also provided.

  本研究提供了一种用于鉴定长链核酸中修饰胞嘧啶（C）的位置和相位的方法。在一些实施方案中，该方法可包括 (a) 将含有至少一个 C 和/或至少一个修饰 C 的核酸样本的第一部分与 DNA 葡糖基转移酶和胞苷脱氨酶反应，以产生第一产物，并选择性地将样本的第二部分与二氧酶和胞苷脱氨酶反应，以产生第二产物，以及；(b) 将(a)中获得的第一和第二产物或其扩增产物的序列相互比较和/或与未经处理的参考序列比较，以确定初始核酸片段中哪些 Cs 被修饰。还提供了一种修饰的 TET 甲基胞嘧啶二氧 化酶，它能更有效地将甲基胞嘧啶转化为羧甲基胞嘧啶。
• COMPOSITIONS AND METHODS FOR DETERMINING MODIFIED CYTOSINES BY SEQUENCING
  申请人：New England Biolabs, Inc.

  公开号：EP3368688A1

  公开（公告）日：2018-09-05
• Compositions and Methods for Determining Modified Cytosines by Sequencing
  申请人：New England Biolabs, Inc.

  公开号：US20180312914A1

  公开（公告）日：2018-11-01

  A method for identifying the location and phasing of modified cytosines (C) in long stretches of nucleic acids is provided. In some embodiments, the method may comprise (a) reacting a first portion of a nucleic acid sample containing at least one C and/or at least one modified C with a DNA glucosyltransferase and a cytidine deaminase to produce a first product and optionally reacting a second portion of the sample with a dioxygenase and a cytidine deaminase to produce a second product and; (b) comparing the sequences from the first and optionally the second product obtained in (a), or amplification products thereof, with each other and/or an untreated reference sequence to determine which Cs in the initial nucleic acid fragment are modified. A modified TET methylcytosine dioxygenase that is more efficient at converting methylcytosine to carboxymethylcytosine is also provided.
• [EN] COMPOSITIONS AND METHODS FOR DETERMINING MODIFIED CYTOSINES BY SEQUENCING<br/>[FR] COMPOSITIONS ET PROCÉDÉS DE DÉTERMINATION DE CYTOSINES MODIFIÉES PAR SÉQUENÇAGE
  申请人：NEW ENGLAND BIOLABS INC

  公开号：WO2017075436A1

  公开（公告）日：2017-05-04

  A method for identifying the location and phasing of modified cytosines (C) in long stretches of nucleic acids is provided. In some embodiments, the method may comprise (a) reacting a first portion of a nucleic acid sample containing at least one C and/or at least one modified C with a DNA glucosyltransferase and a cytidine deaminase to produce a first product and optionally reacting a second portion of the sample with a dioxygenase and a cytidine deaminase to produce a second product and; (b) comparing the sequences from the first and optionally the second product obtained in (a), or amplification products thereof, with each other and/or an untreated reference sequence to determine which Cs in the initial nucleic acid fragment are modified. A modified TET methylcytosine dioxygenase that is more efficient at converting methylcytosine to carboxymethylcytosine is also provided.