1-{[2-Amino-4-(iodoacetyl)benzoyl]oxy}pyrrolidine-2,5-dione | 128906-09-2

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: 1-{[2-Amino-4-(iodoacetyl)benzoyl]oxy}pyrrolidine-2,5-dione
• 英文别名: (2,5-dioxopyrrolidin-1-yl) 2-amino-4-(2-iodoacetyl)benzoate
• CAS: 128906-09-2
• 化学式: C₁₃H₁₁IN₂O₅
• 分子量: 402.14
• InChiKey: USTTUIOKBUVOAL-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.1
• 重原子数：
  21
• 可旋转键数：
  5
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.23
• 拓扑面积：
  107
• 氢给体数：
  1
• 氢受体数：
  6

文献信息

• Improved method of conjugating metallothionein to biologically active molecules
  申请人：THE DU PONT MERCK PHARMACEUTICAL COMPANY

  公开号：EP0196669A2

  公开（公告）日：1986-10-08

  A process for producing a trace-labeled conjugate of metallothionein and biologically active molecule comprising the sequential steps of: conjugating the BAM to a heterobifunctional crosslinking agent; and conjugating the resulting product of with metallothionein where at least a portion of the metal in metallothionein is a tracer metal.

  一种生产金属硫蛋白和生物活性分子的痕量标记共轭物的工艺，包括以下连续步骤：将 BAM 与杂官能团交联剂共轭；以及将所得产物与金属硫蛋白共轭，其中金属硫蛋白中的金属至少有一部分是示踪金属。
• Up-converting reporters for biological and other assays using laser excitation techniques
  申请人：SRI INTERNATIONAL

  公开号：EP0723146A1

  公开（公告）日：1996-07-24

  The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labelling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical environment sampling techniques.

  本发明提供了通过用上转换标签标记探针分子，对生物大分子等分析物进行灵敏检测的方法、组合物和装置。上转换标签吸收来自照明源的辐射，并以一个或多个较高频率发射辐射，从而提高信噪比，并从根本上消除背景样品自发荧光。这些方法、组合物和装置适用于多种分析物的灵敏检测和各种临床环境采样技术。
• Stable polymerized hemoglobin blood-substitute
  申请人：BIOPURE CORPORATION

  公开号：EP1093720A1

  公开（公告）日：2001-04-25

  A method for producing a stable polymerized hemoglobin blood-substitute from blood characterized in the use of a chromatographic column, is disclosed. The method of this invention includes mixing blood with an anticoagulant to form a blood solution, washing the red blood cells in the blood solution and then separating the washed red blood cells from the white blood cells. This method also includes disrupting the red blood cells to release hemoglobin and form a hemoglobin solution, which is then treated by high performance liquid chromatography to form a hemoglobin eluate. The hemoglobin eluate is then deoxygenated, contacted with a first reducing agent to form an oxidation-stabilized deoxygenated hemoglobin solution, and mixed with a cross-linking agent to form a polymerization reaction mixture, which is then polymerized. The polymerized hemoglobin solution is then diafiltered with a physiologic solution and with a reducing agent, whereby the polymerized hemoglobin solution is made physiologically acceptable, and whereby the reducing agent scavenges oxygen, to form a stable polymerized hemoglobin blood-substitute which is then packaged and stored in an atmosphere substantially free of oxygen. Compositions made by the methods are also disclosed, as are methods of therapeutically, or prophylactically, treating a vertebrate to increase tissue oxygenation, or prevent oxygen depletion, in tissue of the vertebrate.

  本发明公开了一种从血液中生产稳定聚合血红蛋白血液替代物的方法，其特点是使用色谱柱。本发明的方法包括将血液与抗凝剂混合形成血液溶液，在血液溶液中洗涤红细胞，然后将洗涤后的红细胞与白细胞分离。该方法还包括破坏红细胞以释放血红蛋白，形成血红蛋白溶液，然后用高效液相色谱法处理，形成血红蛋白洗脱液。然后将血红蛋白洗脱液脱氧，与第一还原剂接触，形成氧化稳定的脱氧血红蛋白溶液，再与交联剂混合，形成聚合反应混合物，然后进行聚合。然后用生理溶液和还原剂对聚合的血红蛋白溶液进行重过滤，从而使聚合的血红蛋白溶液成为生理上可接受的溶液，并使还原剂清除氧气，形成稳定的聚合血红蛋白血液替代物，然后将其包装并储存在基本上不含氧的环境中。此外，还公开了通过上述方法制成的组合物，以及对脊椎动物进行治疗或预防性治疗的方法，以提高该脊椎动物组织的含氧量或防止氧气耗尽。
• Mass spectrometric detection of polypeptides
  申请人：SEQUENOM, INC.

  公开号：EP1296143A2

  公开（公告）日：2003-03-26

  A process for determining the identity of a target polypeptide using mass spectroscopy is provided. Depending on the target polypeptide to be identified, a process as disclosed can be used, for example, to diagnose a genetic disease or chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogenic organism; or for determining identity or heredity.

  本发明提供了一种利用质谱确定目标多肽身份的方法。根据待鉴定的目标多肽，所公开的过程可用于诊断遗传疾病或染色体异常、易患疾病或病症或感染病原体；或用于确定身份或遗传。
• Methods of nucleic acid amplification and sequencing
  申请人：Solexa Ltd.

  公开号：EP1634963A1

  公开（公告）日：2006-03-15

  Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5' end an oligonucleotide sequence Y and at the 3' end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5' end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5' end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5' ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods are also described.

  对至少一种核酸进行扩增和测序的方法，包括以下步骤： (1) 形成至少一个核酸模板，该模板包含待扩增或测序的核酸，其中所述核酸的 5' 端包含寡核苷酸序列 Y，3' 端包含寡核苷酸序列 Z，此外，核酸的 5' 端还带有将核酸附着到固体支持物上的装置； (2) 在有固体支持物的情况下，将所述核酸模板与一种或多种菌落引物 X 混合，菌落引物 X 可与寡核苷酸序列 Z 杂交，其 5'端带有将菌落引物附着到固体支持物上的装置，从而使核酸模板和菌落引物的 5'端都与固体支持物结合； (3) 对结合的模板进行一个或多个核酸扩增反应，从而产生核酸菌落，并对产生的一个或多个核酸菌落进行至少一个步骤的序列测定。此外，还介绍了用于这些方法的固体支持物、试剂盒和装置。