N²-isobutyrylguanosine

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: N²-isobutyrylguanosine
• 英文别名: N-(2-methylpropanoyl)adenosine；N-[9-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]purin-6-yl]-2-methylpropanamide
• 化学式: C₁₄H₁₉N₅O₅
• 分子量: 337.335
• InChiKey: RSLAEWMJDPSZPS-AKAIJSEGSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.7
• 重原子数：
  24
• 可旋转键数：
  4
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.57
• 拓扑面积：
  143
• 氢给体数：
  4
• 氢受体数：
  8

反应信息

• 作为产物：
  描述：

  鸟苷 、 异丁酸 在 TEA 吡啶 、 对硝基苯酚 、 N,N'-二环己基碳二亚胺 作用下， 以 1,4-二氧六环 为溶剂， 反应 4.0h， 生成 N²-isobutyrylguanosine
  参考文献：
  名称：

  Polymer supported carbodiimide strategy for the synthesis of N-acylated derivatives of deoxy- and ribo purinenucleosides using active esters

  摘要：

  A cost-effective synthetic strategy has been used for the selective protection of the exocyclic amino function of purine nucleosides. Instead of using the common protecting groups in their chloride or anhydride forms, the less expensive and nontoxic acidic form was chosen. The acids were first activated to an active ester form using DCC and further successfully used for N-acylation of purine nucleosides. The contamination of the N-acylated product with DCU was inconvenient and was avoided by use of polymer supported-carbodiimide that has the additional advantage of reusability. (c) 2005 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2005.07.078

文献信息

• Highly functional short hairpin RNA
  申请人：Vermeulen Annaleen

  公开号：US20060223777A1

  公开（公告）日：2006-10-05

  The present invention provides improved hairpin and fractured hairpin constructs for use in gene silencing through the RNA interference pathway. An exemplary short hairpin polynucleotide for use in gene silencing can include a polynucleotide having from about 42 nucleotides to about 106 nucleotides configured for being processed by Dicer. The polynucleotide can include a first region having from about 19 to about 35 nucleotides, a loop region coupled to the first region, the loop region having from about 4 to about 30 nucleotides, and a second region having from about 19 to about 35 nucleotides and having at least about 80% complementarity to the first region. Optionally, one of the first region or second region can have an overhang having less than about 6 nucleotides. Also, the short hairpin can be formed of a plurality of polynucleotides that cooperate to form a hairpin structure.

  本发明提供了通过 RNA 干扰途径用于基因沉默的改良发夹和断裂发夹构建体。用于基因沉默的示例性短发夹多核苷酸可包括具有约 42 个核苷酸至约 106 个核苷酸的多核苷酸，该多核苷酸被配置为可被 Dicer 处理。多核苷酸可包括具有约 19 至约 35 个核苷酸的第一区段、与第一区段相连的环状区段（环状区段具有约 4 至约 30 个核苷酸）以及具有约 19 至约 35 个核苷酸并与第一区段具有至少约 80% 互补性的第二区段。可选地，第一区域或第二区域之一可具有少于约 6 个核苷酸的悬垂。此外，短发夹可以由多个多核苷酸组成，这些多核苷酸合作形成发夹结构。
• EP1608733A4
  申请人：——

  公开号：EP1608733A4

  公开（公告）日：2008-02-27
• MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCE
  申请人：Dharmacon, Inc.

  公开号：EP1608733A2

  公开（公告）日：2005-12-28
• COMPOSITIONS AND METHODS FOR INHIBITING GENE SILENCING BY RNA INTERFERENCE
  申请人：GE Healthcare Dharmacon, Inc.

  公开号：EP1986697B1

  公开（公告）日：2016-06-29
• Modified polynucleotides for reducing off-target effects in RNA interference
  申请人：Leake Devin

  公开号：US20050223427A1

  公开（公告）日：2005-10-06

  Methods and compositions for performing RNA interference with decreased off-target effects are provided The methods and compositions permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of modifications to the siRNA. Uniquely modified siRNAs have been developed that reduce off-target effects incurred in gene-silencing. The modifications comprise 2′-O-alkyl or mismatch modification(s) at specific positions on the sense and/or antisense strands.