Synthesis and immunological evaluation of protein conjugates of <i>Neisseria meningitidis</i> X capsular polysaccharide fragments
作者:Laura Morelli、Damiano Cancogni、Marta Tontini、Alberto Nilo、Sara Filippini、Paolo Costantino、Maria Rosaria Romano、Francesco Berti、Roberto Adamo、Luigi Lay
DOI:10.3762/bjoc.10.247
日期:——
A vaccine to prevent infections from the emerging Neisseria meningitidis X (MenX) is becoming an urgent issue. Recently MenX capsular polysaccharide (CPS) fragments conjugated to CRM197 as carrier protein have been confirmed at preclinical stage as promising candidates for vaccine development. However, more insights about the minimal epitope required for the immunological activity of MenX CPS are needed
预防新出现的脑膜炎奈瑟氏球菌X(MenX)感染的疫苗已成为迫在眉睫的问题。最近,在临床前阶段已确认与CRM197作为载体蛋白缀合的MenX荚膜多糖(CPS)片段是疫苗开发的有希望的候选者。但是,需要更多有关MenX CPS免疫活性所需的最小表位的见解。我们在此报告了完全合成的MenX CPS低聚物(单体,二聚体和三聚体)与CRM197的化学结合。此外,描述了导致MenX CPS片段合成的一些关键步骤的改进。用获得的新糖缀合物免疫后,缀合的三聚体被证明是具有免疫原性活性的最小片段,即使比从天然聚合物中获得的并结合到相同蛋白质上的pentadecamer要低得多。该发现表明,可能需要比三个重复单元长的寡聚物来模拟天然多糖的活性。