(2R,3S,4R,5R)-2-(羟基甲基)-5-(6-亚氨基-3-甲基嘌呤-9-基)四氢呋喃-3,4-二醇 | 72055-62-0

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 中文名称: (2R,3S,4R,5R)-2-(羟基甲基)-5-(6-亚氨基-3-甲基嘌呤-9-基)四氢呋喃-3,4-二醇
• 中文别名: β.-丙氨酸,N-(2-氨基乙基)-,盐,单钠聚合1,4-丁二醇,1,6-二异氰酸基己烷,1,3-二异氰酸基甲基苯和己二酸
• 英文名称: 3-methyladenosine
• 英文别名: 3-Methyladenosin；(2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-imino-3-methylpurin-9-yl)oxolane-3,4-diol
• CAS: 72055-62-0
• 化学式: C₁₁H₁₅N₅O₄
• 分子量: 281.271
• InChiKey: RIFDKYBNWNPCQK-IOSLPCCCSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.9
• 重原子数：
  20
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.55
• 拓扑面积：
  127
• 氢给体数：
  4
• 氢受体数：
  6

文献信息

• LIPID FORMULATIONS FOR DELIVERY OF MESSENGER RNA
  申请人：Translate Bio, Inc.

  公开号：EP3060257B1

  公开（公告）日：2021-02-24
• MUTAGENIZING INTRACELLULAR NUCLEIC ACIDS
  申请人：Biological Research Centre, Hungarian Academy of Sciences

  公开号：EP3526327A1

  公开（公告）日：2019-08-21
• [EN] MUTAGENIZING INTRACELLULAR NUCLEIC ACIDS<br/>[FR] MUTAGÉNISATION D'ACIDES NUCLÉIQUES INTRACELLULAIRES
  申请人：BIOLOGICAL RES CENTRE HUNGARIAN ACADEMY OF SCIENCES

  公开号：WO2018108987A1

  公开（公告）日：2018-06-21

  A method of in vivo mutagenesis of a preselected target region (PTR) of an intracellular DNA within a cell culture, which PTR comprises at least one sequence of interest (SOI) which is at least 60 nucleobases long, the method comprising: a) providing a pool of partially overlapping single stranded DNA (ssDNA) oligonucleotides which upon alignment form a continuous sequence that is complementary to the SOI, wherein the pool contains a diversity of mutagenizing oligonucleotides covering nucleobase mismatches at every position of said SOI and combination of said nucleobase mismatches, wherein each mutagenizing oligonucleotide is hybridizing with the PTR and comprises at least one mismatching nucleobase up to 20% mismatching nucleobases, compared to the SOI; b) integrating the pool into said intracellular DNA by homologous recombination and inducing in vivo mutagenesis of the intracellular DNA through hybridizing the oligonucleotides to the PTR, thereby producing a cell library comprising a repertoire of variant cells covering point mutations at every position and combinations of said point mutations within said PTR.