申请人:Seminis Vegetable Seeds, Inc.
公开号:EP2708607A2
公开(公告)日:2014-03-19
The present invention relates to a method for determining the genotype of a Cruciferous vegetable plant for a plant with an increased glucosinolate level, comprising obtaining a sample of nucleic acids from said plant or a portion thereof and detecting in said nucleic acids a polymorphism at the Myb28 locus that is genetically linked to an increased glucosinolate level. The polymorphism may comprises at least one of: a) a single nucleotide polymorphism (SNP) at a position corresponding to nucleotide 83, 136, 226, 563, 610, 830, 995, 1116, 1513, 1577, 1606, 1620, 1825, 1863, 1877 or 2026 of SEQ ID NO: 1, or b) a polymorphism in the number of nucleotides present between nucleotides 323 and 332, between nucleotides 521 and 524, between nucleotides 783 and 786, between nucleotides and 909 and 914, between nucleotides 1365 and 1369, between 1811 and 1821, or between nucleotides 2046 and 2056 of SEQ ID NO: 1, or c) a polymorphism in the number of nucleotides present between nucleotides 836 and 837, between nucleotides 867 and 868, or between nucleotides 943 and 944 of SEQ ID NO: 1.
本发明涉及一种确定十字花科蔬菜植物基因型的方法,该方法用于确定葡萄糖苷酸水平升高的植物,包括从所述植物或其部分获取核酸样品,并在所述核酸中检测与葡萄糖苷酸水平升高有遗传联系的Myb28基因座上的多态性。该多态性可包括以下至少一种:a)与 SEQ ID NO.1 的核苷酸 83、136、226、563、610、830、995、1116、1513、1577、1606、1620、1825、1863、1877 或 2026 对应位置的单核苷酸多态性 (SNP),或 b) 与 SEQ ID NO.1 的核苷酸 83、136、226、563、610、830、995 对应位置的多态性:1的核苷酸 323 和 332 之间、核苷酸 521 和 524 之间、核苷酸 783 和 786 之间、核苷酸 909 和 914 之间、核苷酸 1365 和 1369 之间、核苷酸 1811 和 1821 之间或核苷酸 2046 和 2056 之间存在的核苷酸数目的多态性,或 c) SEQ ID NO:1 的核苷酸 836 和 837 之间、核苷酸 867 和 868 之间或核苷酸 943 和 944 之间的核苷酸数目的多态性。