2-硝基苯基-ALPHA-D-葡萄糖吡喃糖苷 | 56193-44-3

• 分子结构分类: 有机化合物 - 有机氧化合物
• 中文名称: 2-硝基苯基-ALPHA-D-葡萄糖吡喃糖苷
• 英文名称: o-nitrophenyl-α-D-galactopyranoside
• 英文别名: o-nitrophenyl α-D-glucopyranoside；(2-nitro-phenyl)-α-D-glucopyranoside；(2-Nitro-phenyl)-α-D-glucopyranosid；o-Nitrophenyl-α-D-glucopyranosid；2-Nitrophenol-α-D-glucosid；o-Nitrophenyl-β-glucosid；2-Nitrophenyl a-D-glucopyranoside；(2R,3S,4S,5R,6R)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
• CAS: 56193-44-3
• 化学式: C₁₂H₁₅NO₈
• 分子量: 301.253
• InChiKey: KUWPCJHYPSUOFW-ZIQFBCGOSA-N

物化性质

• 熔点：
  186-188 °C
• 沸点：
  572.4±50.0 °C(Predicted)
• 密度：
  1.599±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -1.2
• 重原子数：
  21
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  145
• 氢给体数：
  4
• 氢受体数：
  8

安全信息

• 储存条件：
  室温

制备方法与用途

2-硝基苯基α-D-吡喃葡萄糖苷是β-葡萄糖苷酶的底物。

反应信息

• 作为反应物：
  描述：

  2-硝基苯基-ALPHA-D-葡萄糖吡喃糖苷 在 Ganoderma lucidium α-galactosidase 作用下， 以 various solvent(s) 为溶剂， 生成 D-葡萄糖 、 硝苯酚
  参考文献：
  名称：

  Purification and Characterization of Thermostable α-Galactosidase fromGanoderma lucidum

  摘要：

  通过硫酸铵沉淀以及 DEAE-Sephadex 和 Con A-Sepharose 柱层析，从灵芝新鲜子实体中纯化了 α-半乳糖苷酶。纯化后的酶在聚丙烯酰胺凝胶电泳中呈均匀分布。其 N 端氨基酸序列与醋酸莫蒂尔氏菌 α-半乳糖苷酶相似。经 SDS 聚丙烯酰胺凝胶电泳测定，该酶的分子质量约为 56 kDa；经凝胶过滤柱层析测定，该酶的分子质量约为 249 kDa。最适 pH 值和温度分别为 6.0 和 70°C。该酶在 70°C 下加热 30 分钟完全稳定。它能水解对硝基苯-α-D-吡喃半乳糖苷（Km=0.4 mM），但很少水解邻硝基苯-α-D-吡喃半乳糖苷。它还能水解三聚果糖、棉子糖和水苏糖。该酶催化了反式半乳糖基化反应，合成了三聚果糖。通过各种分析确认了该产物。

  DOI：

  10.1271/bbb.67.1485
• 作为产物：
  描述：

  2-硝基苯基2,3,4,6-四-O-乙酰基-alpha-D-吡喃葡萄糖苷 生成 2-硝基苯基-ALPHA-D-葡萄糖吡喃糖苷
  参考文献：
  名称：

  The Preparation and Rearrangement of Phenylglycosides

  摘要：

  DOI：

  10.1021/ja01255a060

文献信息

• Peptides for inhibition of herpes simplex virus entry
  申请人：——

  公开号：US20020119165A1

  公开（公告）日：2002-08-29

  The invention includes antiherpesviral peptides and method of generating the same.

  这项发明涉及抗疱疹病毒肽和生成方法。
• Stereoselective thioglycoside syntheses. Part 6. Aryl 4-thiomalto-oligosaccharides as chromogenic substrates for kinetic studies with α-amylase
  作者：Michèle Blanc-Muesser、Jacques Defaye、Hugues Driguez、Guy Marchis-Mouren、Christiana Seigner

  DOI：10.1039/p19840001885

  日期：——

  and their corresponding oxygen analogues were used, in comparative assays, as chromogenic substrates with porcine and human pancreatic α-amylases. In both series, enzymic velocity was higher for the maltotrioside derivatives than for the maltodisaccharides. o-Nitrophenyl glycosides behave as better substrates than the corresponding para isomers. Replacement of intersaccharide oxygen atoms by sulphur

  Nucleophitic双分子取代，任一的ö -或p -硝基苯基2,3,6-三- ø -苯甲酰基-4- ö三氟甲基磺酰基α- d -galactopyranoside（1）或（2）与1钠盐在通常的脱保护序列之后，在六甲基磷酰胺中的-硫代-α- D-吡喃葡萄糖得到了邻和对硝基苯基4-硫代-α-麦芽糖苷（7）和（8）。与一个类似的合成方案（1）和4- thiomaltose（的1-α-硫醇盐12）导致Õ-硝基苯基4,4'-二硫代-α-麦芽三糖苷（15）。在比较试验中，将这些4-硫代寡糖及其相应的氧类似物用作猪和人胰腺α-淀粉酶的显色底物。在两个系列中，麦芽三糖苷衍生物的酶促速度均高于麦芽二糖。与相应的对位异构体相比，邻硝基苯基糖苷的底物性能更好。硫取代糖间氧原子会稍微提高米氏常数，但对水解速率有负面影响。因此，4-thiomaltosylpligosaccharides与他们相比有猪胰α淀粉酶
• Sphingolipid derivatives and their methods of use
  申请人：——

  公开号：US20040039212A1

  公开（公告）日：2004-02-26

  Derivatives of sphingolipids of the formula: 1 are provided wherein the substituents are as defined in the specification and wherein there is at least one R 2 substituent in the sphingolipid derivative. The compounds are useful in the treatment of of abnormal cell proliferation, including benign and malignant tumors, the promotion of cell differentiation, the induction of apoptosis, the inhibition of protein kinase C, and the treatment of inflammatory conditions, psoriasis, inflammatory bowel disease as well as proliferation of smooth muscle cells in the course of development of plaques in vascular tissue. The invention also includes a method for triggering the release of cytochrome c from mitochondria that includes administering an effective amount of a sphingolipid or its derivative or prodrug to a host in need thereof. Further, the invention provides a method for treating bacterial infections, including those that influence colon cancer and other disorders of the intestine, that includes administering an effective amount of one of the active compounds identified herein.

  本发明提供了公式1的鞘脂衍生物，其中取代基如规范中所定义，并且鞘脂衍生物中至少有一个R2取代基。这些化合物可用于治疗异常细胞增殖，包括良性和恶性肿瘤，促进细胞分化，诱导细胞凋亡，抑制蛋白激酶C，以及治疗炎症病症，牛皮癣，炎症性肠病以及在血管组织中形成斑块的平滑肌细胞增殖。本发明还包括一种触发线粒体细胞色素c释放的方法，包括向需要的宿主中投与有效量的鞘脂或其衍生物或前药。此外，本发明提供了一种治疗细菌感染的方法，包括那些影响结肠癌和其他肠道疾病的有效化合物的投与。
• Solid-phase synthesis of oligosaccharides and glycopeptides using glycosynthases
  申请人：University of British Columbia

  公开号：US20030138880A1

  公开（公告）日：2003-07-24

  The present invention provides materials and methods for the solid-phase synthesis of oligosaccharides and glycopeptides. Such materials and methods include mutant glycosidase enzymes, or “glycosynthases,” chemically-derivatized acceptor molecules, and specific solid support matrices.

  本发明提供了用于寡糖和糖肽固相合成的材料和方法。这些材料和方法包括突变的糖苷酶酶或“糖合酶”、化学衍生的受体分子和特定的固定支持基质。
• Methods and device for detection of microorganisms
  申请人：BioControl Systems, Inc.

  公开号：EP0264221A2

  公开（公告）日：1988-04-20

  A device adapted for containing a volume of growth medium and employed for detection of gas produced by a micro­organism or microorganisms putatively contained within a test sample and added to the growth medium, comprising a primary chamber, a dome defined by the top surface of the primary chamber and communicating therewith, the dome establishing a fluid level and adapted for collection of gas produced by a microorganism within the growth medium, and a fill section adja­cent to and communicating with one end of the primary chamber, the fill section spaced apart from the dome and extending above the fluid level, and further defining an opening for the addi­tion of a test sample is disclosed. The device may be sealed by means of a removable closure. An apparatus consisting of an array of substantially identical devices is also disclosed. In addition, methods of using the device and apparatus for detect­ing and estimating numbers of microorganisms, and for analyzing samples putatively containing a microorganism, are disclosed.

  一种装置，适于容纳一定体积的生长培养基，并用于检测由假定包含在测试样品中并被添加到生长培养基中的一种或多种微生物产生的气体，该装置包括一个主腔室、一个由主腔室顶面限定并与之相通的穹顶、该装置包括主室、一个由主室顶面限定并与之相通的圆顶，圆顶确定一个液面并适于收集生长培养基中微生物产生的气体，以及一个与主室一端相邻并相通的填充部分，填充部分与圆顶间隔开并延伸到液面之上，并进一步确定一个用于添加测试样品的开口。该装置可通过一个可拆卸的封闭装置进行密封。还公开了一种由基本相同的装置阵列组成的装置。此外，还公开了使用该装置和设备检测和估计微生物数量以及分析假定含有微生物的样品的方法。