3'-氨基-3'-脱氧胸苷三磷酸酯 | 90053-16-0

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 中文名称: 3'-氨基-3'-脱氧胸苷三磷酸酯
• 英文名称: 3'-amino-3'-deoxythymidine-5'-triphosphate
• 英文别名: 3'amino-3'deoxythymidinetriphosphate；3'-Amino-2',3'-dideoxythymidine 5'-triphosphate；3'-Deoxy-3'-aminothymidine-5'triphosphate；[[(2S,3S,5R)-3-amino-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
• CAS: 90053-16-0
• 化学式: C₁₀H₁₈N₃O₁₃P₃
• 分子量: 481.187
• InChiKey: FGYGYHFEBIMMKO-XLPZGREQSA-N

物化性质

• 密度：
  1.840±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -7.1
• 重原子数：
  29
• 可旋转键数：
  8
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.6
• 拓扑面积：
  245
• 氢给体数：
  6
• 氢受体数：
  14

反应信息

• 作为产物：
  描述：

  Methylene-AzddTTP 在 palladium on activated charcoal 氢气 作用下， 以 甲醇 、 水 为溶剂， 20.0 ℃ 、101.32 kPa 条件下， 反应 2.5h， 以74%的产率得到3'-氨基-3'-脱氧胸苷三磷酸酯
  参考文献：
  名称：

  Synthesis of haptens for the development of a solid-phase immobilized epitope-immunoassay (SPIE-IA) of AZT-TP

  摘要：

  The synthesis of the reduced form of AZT-triphosphate, 3'-amino-3'-deoxythymidine-5'-triphosphate, and of its stable analog, 3'-amino-3'-deoxythymidine-5'-(alpha;beta:beta,gamma-bis-methylene)triphosphate, is described. AMT-TP and AMT-PCPCP were prepared to develop a specific immunometric assay for AZT-TP. (C) 1999 Elsevier Science Ltd. All rights reserved.

  DOI：

  10.1016/s0040-4039(99)00785-6

文献信息

• Macromolecular Nucleotide Compounds and Methods for Using the Same
  申请人：Cherkasov Dmitry

  公开号：US20100093992A1

  公开（公告）日：2010-04-15

  The invention relates to the structure, production and use of modified nucleotide and nucleoside building blocks, (nuc-macromolecules).

  这项发明涉及修饰核苷酸和核苷建构模块（核酸大分子）的结构、生产和使用。
• Nucleic acid labeling compounds
  申请人：McGall H. Glenn

  公开号：US20050003496A1

  公开（公告）日：2005-01-06

  Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. Methods for making such heterocyclic compounds are also disclosed. The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.

  揭示了含有杂环衍生物的核酸标记化合物。还公开了制备这种杂环化合物的方法。这些标记化合物适用于通过酶的附着，无论是末端还是内部，提供核酸检测的机制。
• Nucleic acid analysis techniques
  申请人：——

  公开号：US20030064364A1

  公开（公告）日：2003-04-03

  The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.

  本发明提供了一种用于鉴定两个或多个样本之间核酸丰度（如表达水平）差异的简化方法。该方法包括提供一个包含大量（如超过 1000 个）任意选择的不同寡核苷酸探针的阵列，其中每个不同探针的序列和位置都是已知的。来自两个或多个样本的核酸样本（如 mRNA）与探针阵列杂交，并检测杂交模式。样本间杂交模式的差异表明这些样本间各种基因表达的差异。本发明还提供了一种对核酸进行末端标记的方法。在一个实施方案中，该方法包括提供核酸，提供标记的寡核苷酸，然后将寡核苷酸酶联到核酸上。因此，例如，当核酸是 RNA 时，可使用 RNA 连接酶将标记的寡核苷酸连接到核酸上。在另一个实施方案中，末端标记可以通过提供核酸、提供标记的核苷三磷酸酯以及使用末端转移酶将核苷三磷酸酯连接到核酸上来完成。
• Wojczcwski; Faulstich; Engels, Nucleosides and Nucleotides, 1997, vol. 16, # 5-6, p. 751 - 754
  作者：Wojczcwski、Faulstich、Engels

  DOI：——

  日期：——
• A facile solid phase synthesis of 2′- and 3′-aminonucleoside triphosphates
  作者：Thomas Schoetzau、Torsten Holletz、Dieter Cech

  DOI：10.1039/cc9960000387

  日期：——

  A convenient and time-saving solid phase synthesis of different 2'- and 3'-amino-functionalized nucleoside-5'-triphosphates using polymer bounded triphenylphosphine is described.