羟基甲基脱氧尿苷三磷酸酯 | 14167-70-5

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 中文名称: 羟基甲基脱氧尿苷三磷酸酯
• 英文名称: 5-hydroxymethyl-2'-deoxyuridine-5'-triphosphate
• 英文别名: HOMedUTP；5-Hydroxymethyldeoxyuridin-5'-triphosphat；α-hydroxy-O^5'-(tetrahydroxy-[1]triphosphoryl)-thymidine；Hydroxymethyldeoxyuridine triphosphate；[hydroxy-[[(2R,3S,5R)-3-hydroxy-5-[5-(hydroxymethyl)-2,4-dioxopyrimidin-1-yl]oxolan-2-yl]methoxy]phosphoryl] phosphono hydrogen phosphate
• CAS: 14167-70-5
• 化学式: C₁₀H₁₇N₂O₁₅P₃
• 分子量: 498.171
• InChiKey: RLHFVRMIEVOHOR-XLPZGREQSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -5.7
• 重原子数：
  30
• 可旋转键数：
  9
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.6
• 拓扑面积：
  259
• 氢给体数：
  7
• 氢受体数：
  15

文献信息

• Sample preparation method
  申请人：Oxford Nanopore Technologies Ltd.

  公开号：US10669578B2

  公开（公告）日：2020-06-02

  The invention relates to an improved method for characterising a template polynucleotide. The method involves using a polymerase to prepare a modified polynucleotide which makes it easier to characterise than the template polynucleotide.

  本发明涉及一种表征模板多核苷酸的改进方法。该方法包括使用聚合酶制备修饰的多核苷酸，使其比模板多核苷酸更容易表征。
• Modification of 3′ terminal ends of nucleic acids by DNA polymerase theta
  申请人：Temple University-Of The Commonwealth System of Higher Education

  公开号：US10865396B2

  公开（公告）日：2020-12-15

  The invention provides compositions and methods for modifying the 3′-terminal ends of nucleic acids using DNA polymerase θ terminal transferase activity.

  本发明提供了利用 DNA 聚合酶 θ 末端转移酶活性修饰核酸 3′-末端的组合物和方法。
• Sequencing using non-natural nucleotides
  申请人：Ultima Genomics, Inc.

  公开号：US11326207B2

  公开（公告）日：2022-05-10

  The present disclosure provides methods and systems for nucleic acid sequencing. Such systems and methods may achieve context-independent incorporation, have reduced context-dependence or have context-dependence that is amenable to calibration and modeling. Such systems and methods may also reduce misincorporation.

  本公开提供了核酸测序的方法和系统。这些系统和方法可实现与上下文无关的整合，降低上下文依赖性，或具有可进行校准和建模的上下文依赖性。这些系统和方法还可以减少误结合。
• Methods for amplification and sequencing using thermostable TthPrimPol
  申请人：4basebio SL

  公开号：US11299718B2

  公开（公告）日：2022-04-12

  The present invention is directed to methods for replicating, amplifying, and sequencing of nucleic acids using the thermostable, bifunctional replicase “TthPrimPol” from Thermus thermophilus HB27. The TthPrimPol enzyme is extremely tolerant to alterations of the nucleotides of the template nucleic acid. Therefore, in one aspect the invention discloses methods for replicating, amplifying and sequencing of damaged polynucleotide templates.

  本发明涉及使用来自嗜热菌 HB27 的恒温双功能复制酶 "TthPrimPol "对核酸进行复制、扩增和测序的方法。 TthPrimPol 酶对模板核酸核苷酸的改变具有极强的耐受性。 因此，本发明在一个方面公开了对受损多核苷酸模板进行复制、扩增和测序的方法。
• DNA polymerase theta mutants, methods of producing these mutants, and their uses
  申请人：INSTITUT PASTEUR

  公开号：US11384345B2

  公开（公告）日：2022-07-12

  The invention relates to mutant DNA polymerases of the Pol theta subfamily capable of performing non-templated nucleic acid extension, or of a functional fragment of such a polymerase, methods of producing these mutant DNA polymerases, kits and methods of using these mutant DNA polymerases.

  本发明涉及能够进行非模板核酸延伸的 Pol theta 亚家族的突变 DNA 聚合酶或这种聚合酶的功能片段、生产这些突变 DNA 聚合酶的方法、使用这些突变 DNA 聚合酶的试剂盒和方法。