胞苷5'-四磷酸酯 | 10592-99-1

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 中文名称: 胞苷5'-四磷酸酯
• 英文名称: Cytidine 5'-tetraphosphate
• 英文别名: [[(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [hydroxy(phosphonooxy)phosphoryl] hydrogen phosphate
• CAS: 10592-99-1
• 化学式: C₉H₁₇N₃O₁₇P₄
• 分子量: 563.14
• InChiKey: ZBQGNTDXMLQDQJ-XVFCMESISA-N

物化性质

• 沸点：
  925.0±75.0 °C(Predicted)
• 密度：
  2.63±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -6.7
• 重原子数：
  33
• 可旋转键数：
  10
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  315
• 氢给体数：
  8
• 氢受体数：
  17

反应信息

• 作为产物：
  描述：

  5'-胞苷酸 在 Meiothermus ruber polyphosphate kinase L₁₃₆A 、 magnesium chloride 作用下， 以 aq. buffer 为溶剂， 生成 胞苷-5’-二磷酸 、 胞苷-5’-三磷酸 、 胞苷5'-四磷酸酯
  参考文献：
  名称：

  10.1002/cctc.202400181

  摘要：

  AbstractPolyphosphate kinases (PPKs) are valuable biocatalysts for ATP cofactor regeneration as well as for the phosphorylation of non‐canonical nucleotides. The versatility of PPKs in the latter application is defined by their substrate scope. In this study, we investigate the substrate spectrum of the PPK2‐III enzyme from Meiothermus ruber (MrPPK) for the conversion of canonical and non‐canonical (deoxy)‐ribonucleotides. The enzyme shows high substrate promiscuity for purine and to minor degree pyrimidine substrates, with an overall preference for the native substrate AMP. With structure guided rational amino acid exchanges in the active site, we produced an MrPPK variant with improved activity for a broad variety of purine nucleotides. While the preference for AMP is lost, conversion of nucleotides without a 6‐amino function at the purine moiety is increased. This MrPPK variant is a versatile biocatalyst for the synthesis of non‐canonical nucleotides and could also be useful as a GTP cofactor regeneration system.

  DOI：

  10.1002/cctc.202400181

文献信息

• Synthesis and compositions of 2'-terminator nucleotides
  申请人：Bodepudi Veeraiah

  公开号：US20050037991A1

  公开（公告）日：2005-02-17

  The invention provides compositions the comprise nucleotides and/or nucleosides having blocking groups at 2′-positions of sugar moieties. Methods of synthesizing these nucleic acids are also provided.

  该发明提供了含有在糖基的2′-位置具有阻断基团的核苷酸和/或核苷的组合物。同时还提供了合成这些核酸的方法。
• Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides
  申请人：Bodepudi Veeraiah

  公开号：US20070219361A1

  公开（公告）日：2007-09-20

  The invention provides methods of producing blocked oligonucleotides that include 2′-terminator nucleotides. These blocked oligonucleotides are used primers and probes in a variety of nucleic acid technologies. Related kits are also provided.

  这项发明提供了生产包含2'-终止核苷酸的阻断寡核苷酸的方法。这些阻断寡核苷酸可用作各种核酸技术中的引物和探针。此外还提供了相关的试剂盒。
• SYNTHESIS AND COMPOSITIONS OF 2 -TERMINATOR NUCLEOTIDES
  申请人：Roche Diagnostics GmbH

  公开号：EP1644394B1

  公开（公告）日：2018-05-09
• 2'-TERMINATOR NUCLEOTIDE-RELATED METHODS AND SYSTEMS
  申请人：Roche Diagnostics GmbH

  公开号：EP1641943A2

  公开（公告）日：2006-04-05
• 2'-TERMINATOR RELATED PYROPHOSPHOROLYSIS ACTIVATED POLYMERIZATION
  申请人：Roche Diagnostics GmbH

  公开号：EP2076606A2

  公开（公告）日：2009-07-08