A system for rapidly determining the presence or quantity of coliform bacteria in a water sample using the enzymes β-D-galactosidase and β-D-glucuronidase. The system includes a first filter means for separating bacteria from the sample and a broth for culturing the bacteria including an inducing agent for inducing enzyme production. A second filter means is used to separate the cultured bacteria from the broth. A lysing agent is exposed to the bacteria on the second filter and incubated with a chemiluminogenic substrate of the enzyme to produce a chemiluminescent product. Light emission is initiated from the second filter means and the emitted light is detected or measured directly from the second filter means using a luminometer adapted to receive the second filter means. The system is especially effective at improving the sensitivity and specificity of the assay by increasing the signal received from encapsulated target organisms and reducing the interference from non-target organisms that may be present in the sample.
一种利用酶β-
D-半乳糖苷酶和β-
D-葡萄糖醛酸酶快速测定
水样中大肠菌群的存在或数量的系统。该系统包括用于从样品中分离细菌的第一过滤装置和用于培养细菌的肉汤,肉汤中包括用于诱导产酶的诱导剂。第二个过滤装置用于将培养的细菌与肉汤分离。裂解剂与第二过滤器上的细菌接触,并与酶的
化学发光底物孵育,以产生
化学发光产物。光从第二滤器发出,使用适合接收第二滤器的发光仪直接从第二滤器检测或测量发出的光。该系统通过增加从封装的目标
生物体接收到的信号,减少可能存在于样品中的非目标
生物体的干扰,在提高检测灵敏度和特异性方面特别有效。