1,3-Diphenyl-5-p-Tolyl tetrazolium | 99125-03-8

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 唑类
• 英文名称: 1,3-Diphenyl-5-p-Tolyl tetrazolium
• 英文别名: 2-(4-Methylphenyl)-3,5-diphenyltetrazol-2-ium
• CAS: 99125-03-8
• 化学式: C₂₀H₁₇N₄
• 分子量: 313.382
• InChiKey: QDQHUDINLICEPV-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  6.7
• 重原子数：
  24
• 可旋转键数：
  3
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.05
• 拓扑面积：
  34.6
• 氢给体数：
  0
• 氢受体数：
  2

反应信息

• 作为产物：
  描述：

  对甲苯基四唑红甲臢 在 thallium(III) acetate 、 sodium chloride 作用下， 以 水 、 溶剂黄146 为溶剂， 生成 1,3-Diphenyl-5-p-Tolyl tetrazolium
  参考文献：
  名称：

  Balakrishnan, R.; Srinivasan, V. S.; Venkatasubramanian, N., Indian Journal of Chemistry - Section B Organic and Medicinal Chemistry, 1981, vol. 20, # 5, p. 404 - 406

  摘要：

  DOI：

文献信息

• Method of measuring substance in sample using a redox reaction
  申请人：Kyoto Daiichi Kagaku Co., Ltd.

  公开号：US20020025546A1

  公开（公告）日：2002-02-28

  A highly reliable method of measuring an analyte in a sample using a redox reaction. In this method, a tetrazolium compound is added to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance in the sample, then a reducing substance or an oxidizing substance derived from the analyte is formed, the quantity of the formed substance derived from the analyte is measured by the redox reaction, and the quantity of the analyte is determined from the quantity of the formed substance derived from the analyte. As the tetrazolium compound, for example, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt can be used.

  一种高度可靠的测量样品中分析物的方法，使用氧化还原反应。在该方法中，在氧化还原反应之前向样品中添加四唑化合物以消除样品中任何还原物质的影响，然后形成从分析物中衍生的还原物质或氧化物质，通过氧化还原反应测量从分析物中衍生的物质的数量，并从中确定分析物的数量。例如，可以使用2-（4-碘苯基）-3-（2,4-二硝基苯基）-5-（2,4-二硫酚基）-2H-四唑盐作为四唑化合物。
• Assay method with the use of redox reaction
  申请人：——

  公开号：US20040063213A1

  公开（公告）日：2004-04-01

  A method of determining an analyte in a sample utilizing a redox reaction is provided, by which a highly reliable determined value can be obtained. In this method, a tetrazolium compound is added to a sample in the presence of a surfactant prior to a redox reaction so as to eliminate an effect of hemoglobin and a hemoglobin degradation product as reducing substances in the sample. Thereafter, a reducing or oxidizing substance derived from an analyte is produced and the amount thereof is determined by the redox reaction. Then, the amount of the analyte in the sample is determined based on the amount of the reducing or oxidizing substance thus determined. According to this method, cloudiness due to the coexistence with the hemoglobin can be prevented by the surfactant and thus an increase in the absorbance due to the cloudiness can be inhibited as shown in FIG. 1 . As the surfactant, polyoxyethylene ether and the like can be used.

  提供了一种利用氧化还原反应确定样品中分析物的方法，可以获得高度可靠的确定值。在这种方法中，在氧化还原反应之前，在表面活性剂的存在下向样品中加入四唑化合物，以消除血红蛋白和血红蛋白降解产物作为还原物质对样品的影响。然后，由分析物产生的还原或氧化物质被产生，并通过氧化还原反应确定其数量。然后，根据确定的还原或氧化物质的数量确定样品中分析物的数量。根据这种方法，由于表面活性剂可以防止与血红蛋白的共存而导致的浑浊，因此可以像图1所示那样抑制由于浑浊而导致的吸光度增加。作为表面活性剂，可以使用聚氧乙烯醚等。
• Method of storing tetrazolium compound, stabilizer for use therein, and tetrazolium compound reagent solution stored by the method
  申请人：——

  公开号：US20040157285A1

  公开（公告）日：2004-08-12

  A method for storing a tetrazolium compound stably is provided. The tetrazolium compound is stored in the presence of sodium azide. The tetrazolium compound (A) and the sodium azide (B) are present at a ratio (A:B) in the range from 1:0.02 to 1:6.2. Furthermore, when the tetrazolium compound is stored as a solution, the concentration of the sodium azide is in the range from 0.08 to 3.2 mmol/L and the concentration of the tetrazolium compound is in the range from 0.5 to 8 mmol/L. As the tetrazolium compound, it is preferable to use 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt.

  提供了一种稳定储存四唑化合物的方法。四唑化合物在存在氮化钠的情况下储存。四唑化合物（A）和氮化钠（B）的比例（A：B）在1：0.02至1：6.2的范围内。此外，当四唑化合物以溶液形式储存时，氮化钠的浓度在0.08至3.2毫摩尔/升的范围内，四唑化合物的浓度在0.5至8毫摩尔/升的范围内。作为四唑化合物，最好使用2-（4-碘苯基）-3-（2,4-二硝基苯基）-5-（2,4-二磺酰苯基）-2H-四唑盐。
• Redox reactions for analyte determination using tetrazolium compounds
  申请人：Kyoto Daiichi Kagaku Co., Ltd.

  公开号：EP1002874A2

  公开（公告）日：2000-05-24

  A highly reliable method of measuring an analyte in a sample using a redox reaction. In this method, a tetrazolium compound is added to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance in the sample, then a reducing substance or an oxidizing substance derived from the analyte is formed, the quantity of the formed substance derived from the analyte is measured by the redox reaction, and the quantity of the analyte is determined from the quantity of the formed substance derived from the analyte. As the tetrazolium compound, for example, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt can be used.

  一种利用氧化还原反应测量样品中分析物的高度可靠的方法。在这种方法中，在氧化还原反应之前向样品中加入四唑化合物，以消除样品中任何还原性物质的影响，然后形成由被分析物衍生的还原性物质或氧化性物质，通过氧化还原反应测量由被分析物衍生的已形成物质的数量，并根据由被分析物衍生的已形成物质的数量确定被分析物的数量。例如，可以使用 2-(4-碘苯基)-3-(2,4-二硝基苯基)-5-(2,4-二磺酸苯基)-2H-四唑鎓盐作为四唑化合物。
• METHOD OF QUANTIFYING HEMOGLOBIN AND METHOD OF MEASURING GLYCATION RATIO OF HEMOGLOBIN
  申请人：ARKRAY, Inc.

  公开号：EP1329721A1

  公开（公告）日：2003-07-23

  A method of determining Hb is provided, by which an amount of Hb can be determined easily and accurately without fear of damage to the environment. Hemoglobin in a sample is denatured with a tetrazolium compound to give denatured hemoglobin, and an amount of an optical change in the sample is measured at an absorption wavelength specific to the denatured hemoglobin. Using the amount of the optical change thus measured, an amount of the hemoglobin in the sample can be determined. The amount of the optical change preferably is measured at a wavelength in a range from 520 to 670 nm. According to this method, an amount of Hb can be determined with high accuracy as shown in FIG. 1.

  本发明提供了一种测定血红蛋白的方法，通过这种方法可以方便、准确地测定血红蛋白的含量，而不必担心对环境造成破坏。用四唑化合物使样品中的血红蛋白变性，得到变性血红蛋白，然后在变性血红蛋白的特定吸收波长处测量样品中的光变化量。根据测量到的光变化量，可以确定样品中血红蛋白的含量。光学变化量最好在 520 至 670 纳米范围内的波长下测量。如图 1 所示，根据这种方法可以高精度地测定血红蛋白的含量。