6-Chloro-3-indolyl-beta-D-galactopyranoside

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: 6-Chloro-3-indolyl-beta-D-galactopyranoside
• 英文别名: (2S,3R,4S,5S,6R)-2-[(6-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
• 化学式: C₁₄H₁₆ClNO₆
• 分子量: 329.73
• InChiKey: OQWBAXBVBGNSPW-RKQHYHRCSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.5
• 重原子数：
  22
• 可旋转键数：
  3
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.43
• 拓扑面积：
  115
• 氢给体数：
  5
• 氢受体数：
  6

文献信息

• Novel alizarin-based chromogenic substrates, their uses and composition containing same
  申请人：——

  公开号：US20030082667A1

  公开（公告）日：2003-05-01

  The invention concerns a novel chromogenic substrate based on Alizarin. The invention also concerns the uses to which this substrate can be put, and a formulation containing such a substrate. It consists therefore of a substrate to detect the presence of an enzyme activity, with the following general formula: 1 in which: the two ketone groups of the central ring can be replaced by one group R 11 (in the upper position), and by OH (in the lower position). R 1 is a target part or H, and R 2 is a target part or H, with at least one out of R 1 and R 2 being a target part, R 3 is H, SO 3 H, Cl, Br, F, I, NO 2 , NH 2 , NR 9 R 10 , or an Acylamino Aminoaryl or Aminoacylamino group of the type NHCOX, with X being an Alkyl, Aryl or Aralkyl group or an a-amino acid residue such as Alanine, R4 is H, SO 3 H, Cl, Br, F, I, NO 2 , NH 2 , NR 9 R 10 , OH or an Acylamino Aminoaryl or Aminoacylamino group of the type NHCOX, with X being an Alkyl, Aryl or Aralkyl group or an &agr;-amino acid such as Alanine, according to a modification, R 3 and R 4 form bonds with one another to create a ring with at least five sides, and preferably six sides. R 5 , R 6 , R 7 and R 8 each consist of one of the following atoms or groups of atoms: H, a halogen (particularly Cl or Br), OH, SO 3 H, or an Alkyl or Alkoxy group, and R 9 a R 10 are independently a Methyl, Alkyl, Aryl, Aralkyl group, or one (either R 9 or R 10 ) is a ring structure (Piperidine, Pyrrolidine, Morpholine, etc.) with the other (either R 10 or R 9 ) being a Hydrogen atom. The invention is particularly applicable in the field of diagnosis.

  这项发明涉及一种基于茜素的新型显色底物。该发明还涉及可以应用此底物的用途，以及含有这种底物的配方。因此，它包括一种用于检测酶活性存在的底物，具有以下一般公式：其中：中心环的两个酮基团可以被一个R11基团（在上部位置）和一个OH基团（在下部位置）取代。R1是目标部分或H，R2是目标部分或H，其中至少有一个是目标部分，R3是H、SO3H、Cl、Br、F、I、NO2、NH2、NR9R10或NHCOX类型的Acylamino Aminoaryl或Aminoacylamino基团，其中X是Alkyl、Aryl或Aralkyl基团或α-氨基酸残基，如丙氨酸，R4是H、SO3H、Cl、Br、F、I、NO2、NH2、NR9R10、OH或NHCOX类型的Acylamino Aminoaryl或Aminoacylamino基团，其中X是Alkyl、Aryl或Aralkyl基团或α-氨基酸，如丙氨酸，根据一种修改，R3和R4相互形成键，创建至少五边形，最好是六边形的环。R5、R6、R7和R8分别由以下原子或原子团组成：H、卤素（特别是Cl或Br）、OH、SO3H或Alkyl或Alkoxy基团，而R9和R10独立地是甲基、Alkyl、Aryl、Aralkyl基团，或者其中之一（R9或R10）是环结构（哌啶、吡咯烷、吗啉等），而另一个（R10或R9）是氢原子。该发明在诊断领域特别适用。
• Novel enzymatic substrates for detecting pseudomonas aeruginosa, compositions containing same and method for detecting said species
  申请人：——

  公开号：US20030044877A1

  公开（公告）日：2003-03-06

  This invention concerns an enzyme substrate consisting of a target part and a marker part, hydrolysis of the substrate leading to separation of the target part from the marker part, and said target part being specific for the enzyme activity being assayed. It also concerns a formulation containing at least one such substrate, and a method for detecting the bacterial species Pseudomonas aeruginosa. The target part of the substrate is specific for &bgr;-alanine aminopeptidase activity and the marker part is a compound which reveals whether a hydrolysis reaction has taken place or not. The invention is particularly applicable in the field of diagnosis.

  本发明涉及一种酶底物，包括目标部分和标记部分，水解该底物导致目标部分与标记部分分离，且所述目标部分特异于正在检测的酶活性。本发明还涉及至少含有这样的底物的配方，以及一种检测细菌种Pseudomonas aeruginosa的方法。该底物的目标部分特异于β-丙氨酸氨肽酶活性，标记部分是一种化合物，用于显示是否发生了水解反应。本发明特别适用于诊断领域。
• Novel chromogenic substrates based on Alizarin, their applications and formulations containing such substrates
  申请人：Armstrong Lyle

  公开号：US20060172364A1

  公开（公告）日：2006-08-03

  An alizarin-based chromogenic substrate, a composition containing the substrate, and its use to detect the presence of an enzymatic activity. The substrate is particularly applicable in the field of biological diagnosis.

  一种基于茜素的显色底物、含有该底物的组合物及其用于检测酶活性的用途。该底物特别适用于生物诊断领域。
• Chromogenic enzyme substrates
  申请人：Burton Michael

  公开号：US20050124556A1

  公开（公告）日：2005-06-09

  This invention provides novel chromogenic enzyme substrates which are indoxyl β-D-ribofuranosides. A process for their production is provided. Methods for detecting β-D-ribofuranosidase activity are given. The advantages of these novel compounds includes: detecting β-D-ribofuranosidase activity with high sensitivity and low substrate concentrations and use with other enzyme indicators in situations where a plurality of enzyme activities is to be visualized simultaneously e.g. for identifying bacteria growth on solid growth media. The synthesis and use of 5-bromo-4-chloro-3-indolyl-β-D-ribofuranoside is exemplified.

  本发明提供了新型的色团酶底物，即吲哚基β-D-核糖苷。同时提供了一种生产这些底物的方法，并给出了检测β-D-核糖苷酶活性的方法。这些新化合物的优点包括：高灵敏度和低底物浓度下检测β-D-核糖苷酶活性，并可与其他酶指示剂一起使用，用于同时可视化多种酶活性的情况，例如用于识别固体培养基上的细菌生长。其中以5-溴-4-氯-3-吲哚基-β-D-核糖苷的合成和使用为例。
• Milieux de culture et d'identification spécifique de différentes espèces de candida et procédés d'analyse
  申请人：bioMérieux

  公开号：EP1536018A2

  公开（公告）日：2005-06-01

  L'invention concerne un milieu de culture et d'identification spécifique de levures comprenant un substrat chromogène ou fluorigène, susceptible d'être hydrolysé par une enzyme du groupe des hexosaminidases et éventuellemnet des glucosidases, et/ou au moins un composé sélectivement inhibiteur de l'activité hexosaminidase de C. tropicalis, et un procédé pour identifier sélectivement C. albicans et/ou C. tropicalis et/ou d'autres Candida, utilisant un tel milieu. La présente invention trouve une application préférentielle dans le domaine du diagnostic biomédical et plus particulièrement en bactériologie.

  本发明涉及一种用于培养和特异性鉴定酵母菌的培养基，该培养基包括一种能被己糖胺 酶和可选的葡糖苷酶水解的显色或荧光底物，和/或至少一种选择性抑制热带念珠菌己糖胺 酶活性的化合物，以及一种使用这种培养基选择性鉴定白念珠菌和/或热带念珠菌和/或其它念珠 菌的方法。 本发明优先应用于生物医学诊断领域，尤其是细菌学领域。