Didesulfo Cyanine 3 Monofunctional Hexanoic Acid Dye | 1353432-80-0

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吲哚及其衍生物
• 英文名称: Didesulfo Cyanine 3 Monofunctional Hexanoic Acid Dye
• 英文别名: 6-[2-[3-(1-ethyl-3,3-dimethylindol-2-ylidene)prop-1-enyl]-3,3-dimethylindol-1-ium-1-yl]hexanoic acid
• CAS: 1353432-80-0；865650-85-7
• 化学式: C₃₁H₃₉N₂O₂
• 分子量: 471.663
• InChiKey: WGLNVGXNVHWNGV-UHFFFAOYSA-O

计算性质

• 辛醇/水分配系数(LogP)：
  6.5
• 重原子数：
  35
• 可旋转键数：
  9
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.42
• 拓扑面积：
  43.6
• 氢给体数：
  1
• 氢受体数：
  3

反应信息

• 作为反应物：
  描述：

  6-aminohexyl 2-acetamido-2-deoxy-β-D-glucopyranoside 、 Didesulfo Cyanine 3 Monofunctional Hexanoic Acid Dye 在 1-羟基苯并三唑 、 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 、 三乙胺 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 12.0h， 以65 mg的产率得到2-(3-(1-(6-((6-(((2R,3R,4R,5S,6R)-3-acetamido-4,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)hexyl)amino)-6-oxohexyl)-3,3-dimethylindolin-2-ylidene)prop-1-en-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium
  参考文献：
  名称：

  葡萄糖-1-磷酸胸苷基转移酶在尿苷5'-二磷酸半乳糖的合成中的应用及其在N-乙酰乙酰半乳糖胺合成中的应用

  摘要：

  在这项研究中，我们描述了野生型细菌胸苷基转移酶（RmlA）直接合成尿苷5'-二磷酸半乳糖（UDP-Gal），自然界中用于合成胸苷5'-二磷酸葡萄糖（TDP-葡萄糖） 。通过使用镁（Mg 2+）作为辅助因子，反应温度为55°C，RmlA可以实现100毫克规模的UDP-Gal合成。此外，RmlA被定点和共价固定在磁性纳米颗粒（MNPa）上。在十次连续的酶法测定中重复使用后，所得RmlA-MNP复合物保留了其活性的几乎95％。此外，脑膜炎奈瑟氏球菌的β-1,4-半乳糖基转移酶（GalT）通过使用intein介导的蛋白质表达系统成功地成功表达和纯化了p53。GalT在25°C下相对稳定，并且在DTT和BSA的存在下其活性得到增强。因此，在依次添加酶和调节反应温度的情况下，使用RmlA和GalT合成N-乙酰基乳糖胺（LacNAc）是可行的。这些结果证明了细菌RmlA在碳水化合物合成中的潜在应用。

  DOI：

  10.1002/adsc.201100402

文献信息

• Dye compounds
  申请人：SFC CO., LTD.

  公开号：US10473666B2

  公开（公告）日：2019-11-12

  The present invention relates to dye compounds represented by Formulae I and II, which are described in the specification. The dye compounds of the present invention have markedly improved quantum yields and emit strong fluorescence compared to existing cyanine dyes. Due to these advantages, the dye compounds of the present invention can find applications in various fields, for example, as probes for various biological systems where optical imaging is required. Particularly, the dye compounds of the present invention can be used as mitotrackers capable of labeling and tracking mitochondria. Therefore, the dye compounds of the present invention can be used to quantitatively image mitochondria in live tissues and cells. Furthermore, the dye compounds of the present invention can be applied as pH probes for measuring the pH of live cells.

  本发明涉及由式I和II表示的染料化合物，这些化合物在说明书中有描述。与现有的青菁染料相比，本发明的染料化合物具有明显改善的量子产率，并且发射强烈的荧光。由于这些优点，本发明的染料化合物可以在各个领域找到应用，例如，作为各种生物系统的探针，其中需要光学成像。特别地，本发明的染料化合物可以用作能够标记和追踪线粒体的有丝分裂追踪剂。因此，本发明的染料化合物可以用于定量成像活体组织和细胞中的线粒体。此外，本发明的染料化合物可以作为用于测量活细胞pH值的pH探针。
• DYE COMPOUND
  申请人：SFC Co., Ltd.

  公开号：EP3309552A1

  公开（公告）日：2018-04-18

  The present invention relates to dye compounds represented by Formulae I and II, which are described in the specification. The dye compounds of the present invention have markedly improved quantum yields and emit strong fluorescence compared to existing cyanine dyes. Due to these advantages, the dye compounds of the present invention can find applications in various fields, for example, as probes for various biological systems where optical imaging is required. Particularly, the dye compounds of the present invention can be used as mitotrackers capable of labeling and tracking mitochondria. Therefore, the dye compounds of the present invention can be used to quantitatively image mitochondria in live tissues and cells. Furthermore, the dye compounds of the present invention can be applied as pH probes for measuring the pH of live cells.

  本发明涉及由式 I 和式 II 所代表的染料化合物，其在说明书中已有描述。与现有的氰基染料相比，本发明的染料化合物明显提高了量子产率，并能发出强烈的荧光。由于这些优点，本发明的染料化合物可以应用于各个领域，例如，作为需要光学成像的各种生物系统的探针。特别是，本发明的染料化合物可用作线粒体追踪剂，能够标记和追踪线粒体。因此，本发明的染料化合物可用于对活组织和细胞中的线粒体进行定量成像。此外，本发明的染料化合物还可用作 pH 探针，用于测量活细胞的 pH 值。
• CYANINE DYES
  申请人：Carter Tim

  公开号：US20100136567A1

  公开（公告）日：2010-06-03

  The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
• DYE COMPOUNDS
  申请人：SFC CO., LTD.

  公开号：US20180203015A1

  公开（公告）日：2018-07-19

  The present invention relates to dye compounds represented by Formulae I and II, which are described in the specification. The dye compounds of the present invention have markedly improved quantum yields and emit strong fluorescence compared to existing cyanine dyes. Due to these advantages, the dye compounds of the present invention can find applications in various fields, for example, as probes for various biological systems where optical imaging is required. Particularly, the dye compounds of the present invention can be used as mitotrackers capable of labeling and tracking mitochondria. Therefore, the dye compounds of the present invention can be used to quantitatively image mitochondria in live tissues and cells. Furthermore, the dye compounds of the present invention can be applied as pH probes for measuring the pH of live cells.
• US7705150B2
  申请人：——

  公开号：US7705150B2

  公开（公告）日：2010-04-27