申请人:Goldberg L. Steven
公开号:US20060100422A1
公开(公告)日:2006-05-11
This invention relates to a recombinant
Pichia pastoris
formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding
P. pastoris
FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant
P. pastoris
FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to
P. pastoris
FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated
P. pastoris
FDH nucleic acids, polypeptides, and antibodies, and methods of using
P. pastoris
FDH in various reactions for industrial or pharmaceutical applications.
本发明涉及一种重组毕赤酵母甲酸脱氢酶(FDH)酶,该酶催化甲酸氧化为二氧化碳,同时还还原烟酰胺腺嘌呤二核苷酸(NAD+)为其还原形式(NADH)。本发明还涉及编码P. pastoris FDH多肽的分离核酸,以及其片段和变体,以及包含这些核酸的载体和宿主细胞。此外,本发明还涉及分离的重组P. pastoris FDH多肽,以及其片段和变体,以及特异性结合P. pastoris FDH多肽、片段或变体的抗体。本发明还涉及获得分离的P. pastoris FDH核酸、多肽和抗体的方法,以及在工业或制药应用中使用P. pastoris FDH的方法。