2-acetamido-4-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-1,6-anhydro-2-deoxy-3-O-[(1R)-1-(amido)ethyl]-β-D-glucopyranose | 1449311-45-8

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: 2-acetamido-4-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-1,6-anhydro-2-deoxy-3-O-[(1R)-1-(amido)ethyl]-β-D-glucopyranose
• CAS: 1449311-45-8
• 化学式: C₁₉H₃₁N₃O₁₁
• 分子量: 477.469
• InChiKey: GGABGZHHRMDPCK-STFZFCBQSA-N

反应信息

• 作为产物：
  描述：

  2-acetamido-4-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-1,6-anhydro-2-deoxy-3-O-[(1R)-1-(methoxycarbonyl)ethyl]-β-D-glucopyranose 在 氨 作用下， 以 甲醇 为溶剂， 反应 8.0h， 以96%的产率得到2-acetamido-4-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-1,6-anhydro-2-deoxy-3-O-[(1R)-1-(amido)ethyl]-β-D-glucopyranose
  参考文献：
  名称：

  Active Site Plasticity within the Glycoside Hydrolase NagZ Underlies a Dynamic Mechanism of Substrate Distortion

  摘要：

  NagZ is a glycoside hydrolase that participates in peptidoglycan (PG) recycling by removing beta-N-acetylglucosamine from PG fragments that are excised from the bacterial cell wall during growth. Notably, the products formed by NagZ, 1,6-anhydroMurNAc-peptides, activate beta-lactam resistance in many Gram-negative bacteria, making this enzyme of interest as a potential therapeutic target. Crystal structure determinations of NagZ from Salmonella typhimurium and Bacillus subtilis in complex with natural substrate, trapped as a glycosyl-enzyme intermediate, and bound to product, define the reaction coordinate of the NagZ family of enzymes. The structures, combined with kinetic studies, reveal an uncommon degree of structural plasticity within the active site of a glycoside hydrolase, and unveil how NagZ drives substrate distortion using a highly mobile loop that contains a conserved histidine that has been proposed as the general acid/base.

  DOI：

  10.1016/j.chembiol.2012.09.016