(2R,5S)-4-amino-2-[6-(dimethylamino)purin-9-yl]-5-(hydroxymethyl)oxolan-3-ol

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: (2R,5S)-4-amino-2-[6-(dimethylamino)purin-9-yl]-5-(hydroxymethyl)oxolan-3-ol
• 化学式: C₁₂H₁₈N₆O₃
• 分子量: 294.31
• InChiKey: RYSMHWILUNYBFW-NVPARVBDSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.5
• 重原子数：
  21
• 可旋转键数：
  3
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.58
• 拓扑面积：
  123
• 氢给体数：
  3
• 氢受体数：
  8

文献信息

• Ribosome-mediated incorporation of peptides and peptidomimetics
  申请人：ARIZON BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

  公开号：US10745705B2

  公开（公告）日：2020-08-18

  Modified ribosomes that were selected using a dipeptidyl-puromycin aminonucleoside are used to mediate site-specific incorporation of one or more peptides and peptidomimetics into protein in a cell free translation system. In addition, new fluorescent dipeptidomimetics have been synthesized and incorporated into proteins, as well as modified proteins containing one or more non-naturally occurring dipeptides.

  在细胞自由翻译系统中，使用二肽-尿霉素氨基核苷酸筛选出的修饰核糖体可用于介导一种或多种肽和拟肽定点结合到蛋白质中。此外，还合成了新的荧光二肽模拟物，并将其加入蛋白质以及含有一种或多种非天然二肽的修饰蛋白质中。
• Detection of protein expression in vivo using fluorescent puromycin conjugates
  申请人：Starck-Green R. Shelley

  公开号：US20060057069A1

  公开（公告）日：2006-03-16

  Disclosed is a class of reagents for examining protein expression in vivo that does not require transfection, radiolabeling, or the prior choice of a candidate gene. Further, a series of puromycin conjugates was constructed bearing various labeling moieties. These conjugates were readily incorporated into expressed protein products in cell lysates in vitro and efficiently cross cell membranes to function in protein synthesis in vivo as indicated by flow cytometry, selective enrichment studies, and western analysis. The present invention demonstrates that labeled-puromycin conjugates offer a general means to examine protein expression in vivo.

  本研究公开了一类用于检测体内蛋白质表达的试剂，它不需要转染、放射性标记或事先选择候选基因。此外，还构建了一系列带有各种标记分子的嘌呤霉素共轭物。流式细胞术、选择性富集研究和 Western 分析表明，这些共轭物在体外很容易与细胞裂解液中表达的蛋白质产物结合，并能有效地穿过细胞膜，在体内发挥蛋白质合成的功能。本发明表明，标记的金霉素共轭物为检查体内蛋白质表达提供了一种通用方法。
• Ribosome-Mediated Incorporation of Peptides and Peptidomimetics
  申请人：Hecht Sidney

  公开号：US20200370057A1

  公开（公告）日：2020-11-26

  Modified ribosomes that were selected using a dipeptidyl-puromycin aminonucleoside are used to mediate site-specific incorporation of one or more peptides and peptidomimetics into protein in a cell free translation system. In addition, new fluorescent dipeptidomimetics have been synthesized and incorporated into proteins, as well as modified proteins containing one or more non-naturally occurring dipeptides.
• US5705491A
  申请人：——

  公开号：US5705491A

  公开（公告）日：1998-01-06
• US5773603A
  申请人：——

  公开号：US5773603A

  公开（公告）日：1998-06-30