The present invention provides novel rapid growing microorganisms and methods for their use in cloning or subcloning nucleic acid molecules. The rapid growing microorganisms of the present invention form colonies more rapidly than microorganisms typically used in molecular biology and thus provide a significant improvement in in vitro cloning methods used extensively in molecular biology. The rapid growing microorganisms of the invention preferably do not contain detectable levels of bacteriophage genetic material from at least one bacteriophage or in the alternative are resistant to infection by one or more bacteriophage types. The invention also relates to kits and compositions used in the methods of the invention.
本发明提供了新型快速生长微
生物及其用于克隆或亚克隆核酸分子的方法。与
分子生物学中通常使用的微
生物相比,本发明的快速生长微
生物形成菌落的速度更快,因此大大改进了
分子生物学中广泛使用的体外克隆方法。本发明的快速生长微
生物最好不含有可检测到的来自至少一种噬菌体的噬菌体遗传物质,或者对一种或多种类型的噬菌体感染具有抗性。本发明还涉及用于本发明方法的试剂盒和组合物。