5-(pentafluorobenzoylamino)fluorescein di-β-D-glucopyranoside | 209540-64-7

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: 5-(pentafluorobenzoylamino)fluorescein di-β-D-glucopyranoside
• 英文别名: PFBFDβGlu；5-(pentafluorobenzoylamino)fluorescein di-β-D-galactopyranoside；Pfb-fdg；2,3,4,5,6-pentafluoro-N-[3-oxo-3',6'-bis[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]spiro[2-benzofuran-1,9'-xanthene]-5-yl]benzamide
• CAS: 209540-64-7
• 化学式: C₃₉H₃₂F₅NO₁₆
• 分子量: 865.672
• InChiKey: YYMJMZGTQFITLS-QBMVDMLASA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.2
• 重原子数：
  61
• 可旋转键数：
  8
• 环数：
  8.0
• sp3杂化的碳原子比例：
  0.33
• 拓扑面积：
  263
• 氢给体数：
  9
• 氢受体数：
  21

文献信息

• Methods, compositions, and kits for analysis of enzyme activity in cells
  申请人：Graham J. Ronald

  公开号：US20050244907A1

  公开（公告）日：2005-11-03

  In one aspect, the present disclosure relates to methods for detecting an activity of an enzyme in a cell. In some embodiments, the methods include contacting a cell with a liposome containing at least one substrate thereby facilitating introduction of the substrate into the cell. The substrate is capable of producing a detectable light signal when acted on by the enzyme, and the signal is detected. The methods can be used in screening agents that can inhibit or activate an enzyme activity. The methods can also be used in various downstream assays such the detection of interactions between intracellular proteins, screening for variants of an enzyme, and detection of various diseases. Compositions and kits for carrying out the various methods are also provided.

  在一个方面，本公开涉及检测细胞中酶活性的方法。在某些实施方案中，这些方法包括使细胞与含有至少一种底物的脂质体接触，从而促进底物进入细胞。底物在酶的作用下能够产生可检测的光信号，并对信号进行检测。这些方法可用于筛选可抑制或激活酶活性的制剂。这些方法还可用于各种下游检测，如检测细胞内蛋白质之间的相互作用、筛选酶的变体以及检测各种疾病。还提供了用于实施各种方法的组合物和试剂盒。
• Cationic liposomes and methods of use
  申请人：Graham Ronald

  公开号：US20060159738A1

  公开（公告）日：2006-07-20

  Highly efficient cationic liposomes are provided as a system for the delivery to cells of agents or compounds, such as, compounds capable of silencing a target protein and enzyme stubstrates. The cationic liposomes can be used in methods of detecting the inhibition activity or apparent activity of a target protein in a cell, and methods of identifying a protein associated with a pathway, such as, a signal transduction pathway in a cell.

  高效阳离子脂质体是一种向细胞输送药剂或化合物的系统，例如，能够抑制目标蛋白质和酶残基的化合物。阳离子脂质体可用于检测细胞中目标蛋白质的抑制活性或明显活性的方法，以及鉴定与细胞中信号转导途径等途径相关的蛋白质的方法。
• METHODS, COMPOSITIONS, AND KITS FOR ANALYSIS OF ENZYME ACTIVITY IN CELLS
  申请人：Applera Corporation, Applied Biosystems Group

  公开号：EP1704244A2

  公开（公告）日：2006-09-27
• Cationic Liposomes And Method of Use
  申请人：Graham Ronald

  公开号：US20100022001A1

  公开（公告）日：2010-01-28

  Highly efficient cationic liposomes are provided as a system for the delivery to cells of agents or compounds, such as, compounds capable of silencing a target protein and enzyme substrates. The cationic liposomes can be used in methods of detecting the inhibition activity or apparent activity of a target protein in a cell, and methods of identifying a protein associated with a pathway, such as, a signal transduction pathway in a cell.
• US5773236A
  申请人：——

  公开号：US5773236A

  公开（公告）日：1998-06-30