3-[2-[5-[1-(2-Carboxyethyl)-3,3-dimethylindol-1-ium-2-yl]penta-2,4-dienylidene]-3,3-dimethylindol-1-yl]propanoic acid | 160886-45-3

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吲哚及其衍生物
• 英文名称: 3-[2-[5-[1-(2-Carboxyethyl)-3,3-dimethylindol-1-ium-2-yl]penta-2,4-dienylidene]-3,3-dimethylindol-1-yl]propanoic acid
• 英文别名: 3-[2-[5-[1-(2-carboxyethyl)-3,3-dimethylindol-1-ium-2-yl]penta-2,4-dienylidene]-3,3-dimethylindol-1-yl]propanoic acid
• CAS: 160886-45-3
• 化学式: C₃₁H₃₅N₂O₄
• 分子量: 499.63
• InChiKey: PAKOTSWXHOGBNY-UHFFFAOYSA-O

计算性质

• 辛醇/水分配系数(LogP)：
  5.2
• 重原子数：
  37
• 可旋转键数：
  9
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.32
• 拓扑面积：
  80.8
• 氢给体数：
  2
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  O-(2-氨基乙基)-O'-(2-叠氮乙基)九聚乙二醇 、 3-[2-[5-[1-(2-Carboxyethyl)-3,3-dimethylindol-1-ium-2-yl]penta-2,4-dienylidene]-3,3-dimethylindol-1-yl]propanoic acid 在 1-羟基苯并三唑 、 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 、 三乙胺 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 以50%的产率得到
  参考文献：
  名称：

  A Semisynthetic Fluorescent Sensor Protein for Glutamate

  摘要：

  We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor displays a ratiometric change of 1.56. The high sensitivity toward glutamate concentration changes of the sensor and its exclusive extracellular localization make it an attractive tool for glutamate sensing in neurobiology.

  DOI：

  10.1021/ja3002277

文献信息

• Selective Cross-Linking of Interacting Proteins Using Self-Labeling Tags
  作者：Arnaud Gautier、Eiji Nakata、Gražvydas Lukinavičius、Kui-Thong Tan、Kai Johnsson

  DOI：10.1021/ja907818q

  日期：2009.12.16

  selectively cross-link interacting proteins in cell lysate. The amount of trapped complex can be then quantified after SDS gel electrophoresis by in-gel fluorescence scanning. On the basis of a detailed kinetic analysis of the cross-linking reaction, we showed that the cross-linking efficiency can be used as an indicator of interaction between two proteins, allowing thereby the unambiguous identification

  我们设计了允许细胞裂解物中相互作用蛋白选择性交联 (S-CROSS) 的分子，并通过凝胶内荧光扫描对捕获的复合物进行灵敏检测。S-CROSS 需要将假定的相互作用蛋白表达为与 CLIP 标签或 SNAP 标签的融合，这两种蛋白质标签可以用合成探针进行特异性标记。包含通过荧光团连接的两个标签底物的双功能分子用于选择性地交联细胞裂解物中的相互作用蛋白质。然后可以在 SDS 凝胶电泳后通过凝胶内荧光扫描定量捕获复合物的数量。在对交联反应进行详细动力学分析的基础上，我们表明交联效率可以作为两种蛋白质相互作用的指标，从而允许明确识别相互作用的蛋白质对。我们通过选择性交联确认许多相互作用来验证我们的方法，并表明它允许对多个同型和异型蛋白质复合物进行定量和同时分析，以及强弱蛋白质-蛋白质相互作用之间的区别。
• PIGMENT FOR USE WITH PHOTOELECTRIC CONVERSION ELEMENT, AND PHOTOELECTRIC CONVERSION ELEMENT
  申请人：TDK Corporation

  公开号：EP2372829A1

  公开（公告）日：2011-10-05

  A photoelectric conversion device capable of improving conversion efficiency is provided. The photoelectric conversion device includes a work electrode 10, a counter electrode 20, and an electrolyte-containing layer 30. In the work electrode 10, a metal oxide semiconductor layer 12 supporting a dye 13 is provided. The dye 13 contains a cyanine compound. The cyanine compound has heterocyclic skeletons containing indolenine skeletons bonded with both ends of a methine chain, an anchor group introduced to a nitrogen atom contained in the indolenine skeletons, and a phenanthrene skeleton contained in one of the heterocyclic skeletons. In the case where light enters the dye 13, electron injection efficiency to the metal oxide semiconductor layer 12 is improved.

  本文提供了一种能够提高转换效率的光电转换装置。该光电转换装置包括工作电极 10、对电极 20 和含电解质层 30。在工作电极 10 中，有一个支撑染料 13 的金属氧化物半导体层 12。染料 13 含有一种氰化合物。氰基化合物具有杂环骨架，杂环骨架包含与甲基链两端键合的吲哚啉骨架、引入到吲哚啉骨架所含氮原子上的锚基团，以及杂环骨架之一所包含的菲骨架。在光线进入染料 13 的情况下，金属氧化物半导体层 12 的电子注入效率得到提高。
• DYE FOR PHOTOELECTRIC CONVERSION ELEMENT AND PHOTOELECTRIC CONVERSION ELEMENT
  申请人：TDK Corporation

  公开号：EP2330680B1

  公开（公告）日：2013-10-23
• FLUORESCENT PROBE
  申请人：Nagano Tetsuo

  公开号：US20110117666A1

  公开（公告）日：2011-05-19

  A fluorescent probe comprising a compound represented as (Fluorophore A)-S-(Fluorophore B) (Fluorophore A and Fluorophore B are fluorophores which emit fluorescence when they are irradiated with an excitation light of a wavelength of 600 to 950 nm, Fluorophore A has a property that it shows change of fluorescence characteristic before and after a specific reaction with an substance to be measured, and S represents a spacer which connects Fluorophore A and Fluorophore B), which compound shows substantial change in efficiency of fluorescence resonance energy transfer between Fluorophore A and Fluorophore B before and after the specific reaction with the objective substance, wherein Fluorophore A is represented by the following formula (AI): which uses and shows an excitation light wavelength/fluorescence wavelength in the near infrared region, lights of which region show superior biological tissue permeability, and enables measurement of an substance to be measured by the ratio method.
• US8465985B2
  申请人：——

  公开号：US8465985B2

  公开（公告）日：2013-06-18