2,4-dinitrophenyl 1,4-β-D-galactobioside | 200504-60-5

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: 2,4-dinitrophenyl 1,4-β-D-galactobioside
• CAS: 200504-60-5
• 化学式: C₁₈H₂₄N₂O₁₅
• 分子量: 508.393
• InChiKey: AQJZDRFAWVURAR-BQVHMPCVSA-N

反应信息

• 作为反应物：
  描述：

  2,4-dinitrophenyl 1,4-β-D-galactobioside 在 水 作用下， 生成 4-O-β-D-吡喃半乳糖基-D-半乳吡喃糖
  参考文献：
  名称：

  Activity of three β-1,4-galactanases on small chromogenic substrates

  摘要：

  beta-1,4-Galactanases belong to glycoside hydrolase family GH 53 and degrade galactan and arabinogalactan side chains of the complex pectin network in plant cell walls. Two fungal beta-1,4-galactanases from Aspergillus aculeatus, Meripileus giganteus and one bacterial enzyme from Bacillus licheniformis have been kinetically characterized using the chromogenic substrate analog 4-nitrophenyl beta-1,4-D-thiogalactobioside synthesized by the thioglycoligase approach. Values of k(cat)/K-m for this substrate with A. aculeatus beta-1,4-galactanase at pH 4.4 and for M. giganteus beta-1,4-galactanase at pH 5.5 are 333 M (1) s (1) and 62 M (1) s (1), respectively. By contrast the B. licheniformis beta-1,4-galactanase did not hydrolyze 4-nitrophenyl beta-1,4-D-thiogalactobioside. The different kinetic behavior observed between the two fungal and the bacterial beta-1,4-galactanases can be ascribed to an especially long loop 8 observed only in the structure of B. licheniformis beta-1,4-galactanase. This loop contains substrate binding subsites -3 and -4, which presumably cause B. licheniformis beta-1,4-galactanase to bind 4-nitrophenyl -1,4-beta-D-thiogalactobioside non-productively. In addition to their cleavage of 4-nitrophenyl -1,4-beta-D-thiogalactobioside, the two fungal enzymes also cleaved the commercially available 2-nitrophenyl-1,4-beta-D-galactopyranoside, but kinetic parameters could not be determined because of transglycosylation at substrate concentrations above 4 mM. (C) 2011 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.carres.2011.05.017