The invention relates to a process for the biosynthetic production and recovery of [5S,6S]-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylic acid) catalyzed, at increased enzyme activity, at least by an enzyme belonging to the class of aminodeoxyisochorismate (ADIC) synthases and an enzyme belonging to the class of isochorismatases, wherein
(a) the production is carried out fermentatively with a suitable carbon source as starting material in a chorismate overproducing host microorganism, and with an aminodeoxyisochorismate (ADIC) synthase enzyme selected from the group of such ADIC synthase enzymes that are lacking ADIC lyase activity, and
(b) the recovery is carried out by means of concentrating the supernatant of the fermentation to a 2,3-CHA concentration of at least 25 g/l, followed by crystallisation from the said concentrated supernatant.
In a second embodiment, the present invention relates to the use of [5S,6S]-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylic acid (2,3-CHA) as a catalytically active product, in particular as a chiral catalyst.
Finally, the invention relates to the novel zinc complex of 2,3-CHA.
本发明涉及一种
生物合成生产和回收[5S,6S]-6-
氨基-5-羟基
环己-1,3-二烯-1-羧酸的过程,至少由属于
氨基脱氧异
异恶唑酸(ADIC)合成酶类和
异恶唑酸酶类的酶催化,在增加酶活性的情况下,其中(a)生产是在具有咽
氨酸过多的宿主微
生物中,以适当的碳源作为起始材料,利用选择自不具有ADIC裂解酶活性的ADIC合成酶酶类之一的ADIC合成酶酶进行发酵生产的,并且(b)回收是通过将发酵液上清浓缩至至少25g/l的2,3-CHA浓度,然后从所述浓缩上清中结晶实现的。在第二个实施例中,本发明涉及将[5S,6S]-6-
氨基-5-羟基
环己-1,3-二烯-1-羧酸(2,3-CHA)用作催化活性产品,特别是用作手性催化剂。最后,本发明涉及2,3-CHA的新
锌配合物。