The invention provides a novel transferase that acts on a saccharide, as a substrate, composed of at least three sugar units wherein at least three glucose residues on the reducing end are linked α-1,4 so as to transfer the α-1,4 lingages to a α-1, α-1 linkages; a process for producing the transferase; a gene coding for the same; and a process for producing an oligosaccharide by using the same. Also provided are a novel amylase that has a principal activity of acting on a saccharide, as a substrate, composed of at least three sugar units wherein at least three sugar units on the reducing end side are glucose units and the linkage between the first and the second glucose units is α-1, α-1 while the linkage between the second and the third glucose units is α-1,4 so as to liberate α, α-trehalose by hydrolyzing the α-1,4 linkage and another activity of hydrolyzing the α-1,4 linkage within the molecular chain of the substrate and that liberates disaccharides and/or monosaccharides as the principal final products; a process for producing the amylase; a gene coding for the same; and a process for producing α, α-trehalose by using a combination of the transferase and the amylase.
本发明提供了一种新型转移酶,其作用于由至少三个糖单元组成的以糖为底物的
多糖,其中还原端上至少三个
葡萄糖残基以α-1,4键连接,以将α-1,4键转移至α-1,α-1键;一种生产该转移酶的方法;编码该转移酶的
基因;以及使用该转移酶生产
寡糖的方法。本发明还提供了一种新型
淀粉酶,其主要活性作用于由至少三个糖单元组成的以糖为底物的
多糖,其中还原端侧的至少三个糖单元是
葡萄糖单元,第一和第二
葡萄糖单元之间的连接是α-1,α-1键,第二和第三
葡萄糖单元之间的连接是α-1,4键,以通过
水解α-1,4键释放α,α-
海藻糖,并且还具有
水解底物分子链中α-1,4键的另一活性,以释放二糖和/或
单糖作为主要最终产物;一种生产该
淀粉酶的方法;编码该
淀粉酶的
基因;以及使用转移酶和
淀粉酶的组合生产α,α-
海藻糖的方法。