Fluorescein di(beta-D-galactopyranoside)

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: Fluorescein di(beta-D-galactopyranoside)
• 英文别名: 3',6'-bis[[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]spiro[2-benzofuran-3,9'-xanthene]-1-one
• 化学式: C₃₂H₃₂O₁₅
• 分子量: 656.6
• InChiKey: ZTOBILYWTYHOJB-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.2
• 重原子数：
  47
• 可旋转键数：
  6
• 环数：
  7.0
• sp3杂化的碳原子比例：
  0.41
• 拓扑面积：
  234
• 氢给体数：
  8
• 氢受体数：
  15

文献信息

• BRET SENSOR MOLECULES FOR DETECTING HYDROLASES
  申请人：Commonwealth Scientific and Industrial Research Organisation

  公开号：US20210018497A1

  公开（公告）日：2021-01-21

  The present invention relates to bioluminescence resonance energy transfer sensor molecules having the structure R 1 -L-R 2 —B or B—R 2 -L-R 1 , wherein R 1 is a bioluminescent protein, L is a linking element, R 2 is a non-protein acceptor domain and B is a blocking group, and wherein R 2 bound to B comprises a hydrolysable bond which produces a change in BRET when hydrolysed. The invention also discloses a method of detecting a hydrolase by contacting a sample with a molecule B—R 2 , then contacting with a compound R 1 -L or L-R 1 under conditions to cause attaching of R 2 to L, and detecting a change in the BRET ratio. Specifically exemplified sensors comprise luciferase and fluorescein diacetate, which is hydrolysed by an esterase. The invention also discloses luciferase enzymes derived from RLuc8 by removing cysteine residues.
• [EN] BRET SENSOR MOLECULES FOR DETECTING HYDROLASES<br/>[FR] MOLÉCULES DE DÉTECTION DE BRET POUR LA DÉTECTION D'HYDROLASES
  申请人：COMMW SCIENT IND RES ORG

  公开号：WO2019036769A1

  公开（公告）日：2019-02-28

  The present invention relates to bioluminescence resonance energy transfer sensor molecules having the structure R1-L-R2 -B or B- R2-L-R1, wherein R1 is a bioluminescent protein, L is a linking element, R2 is a non-protein acceptor domain and B is a blocking group, and wherein R2 bound to B comprises a hydrolysable bond which produces a change in BRET when hydrolysed. The invention also discloses a method of detecting a hydrolase by contacting a sample with a molecule B-R2, then contacting with a compound R1-L or L-R1 under conditions to cause attaching of R2 to L, and detecting a change in the BRET ratio. Specifically exemplified sensors comprise luciferase and fluorescein diacetate, which is hydrolysed by an esterase. The invention also discloses luciferase enzymes derived from RLuc8 by removing cysteine residues.