oxaloacetate | 27531-88-0

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 酮酸及其衍生物
• 英文名称: oxaloacetate
• 英文别名: 4-Hydroxy-2,4-dioxobutanoate；4-hydroxy-2,4-dioxobutanoate
• CAS: 27531-88-0
• 化学式: C₄H₃O₅
• 分子量: 131.065
• InChiKey: KHPXUQMNIQBQEV-UHFFFAOYSA-M

计算性质

• 辛醇/水分配系数(LogP)：
  0
• 重原子数：
  9
• 可旋转键数：
  2
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  94.5
• 氢给体数：
  1
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  oxaloacetate 在 L-谷氨酸 、 磷酸吡哆醛 作用下， 以 水 为溶剂， 生成 L-天门冬氨酸
  参考文献：
  名称：

  Application of E. Coli aspartate transaminase to amino acid synthesis

  摘要：

  DOI：

  10.1016/s0040-4039(00)96374-3
• 作为产物：
  描述：

  piruvate 、 2,4-二硝基苯肼 、 L-丙氨酸 、 aspartic acid anion II 、 2-氧代-戊二酸离子(2-) 生成 oxaloacetate 、 L-谷氨酸
  参考文献：
  名称：

  Noninvasive Transdermal Systems for Detecting an Analyte in a Biological Fluid and Methods

  摘要：

  本发明涉及非侵入性经皮系统，包括非侵入性经皮贴片和反射计。非侵入性经皮贴片由湿化学组分和干化学组分组成。湿化学组分是一种液体传递介质，以凝胶层的形式从皮肤内或皮肤下的生物流体中提取和液体桥传递感兴趣的分析物到干化学组分中。干化学组分是一种试剂系统，用于与感兴趣的分析物（葡萄糖）相互作用以生成颜色变化。反射计包括一个调制光源，用于发射光线以照亮目标表面，该表面具有一定的颜色和色调，以便光学检测器检测。输出信号经过处理以确定相应的质量测量量。

  公开号：

  US20100049016A1

文献信息

• Altering the Substrate Specificity of Glutamate Dehydrogenase from<i>Bacillus subtilis</i>by Site-Directed Mutagenesis
  作者：Md. Iqbal Hassan KHAN、Hyeung KIM、Hiroyuki ASHIDA、Takahiro ISHIKAWA、Hitoshi SHIBATA、Yoshihiro SAWA

  DOI：10.1271/bbb.69.1802

  日期：2005.1

  The Lys80, Gly82 and Met101 residues of glutamate dehydrogenase from Bacillus subtilis were mutated into a series of single mutants. The wild-type enzyme was highly specific for 2-oxoglutarate, whereas G82K and M101S dramatically switched to increased specificity for oxaloacetate with kcat values 3.45 and 5.68 s-1, which were 265-fold and 473-fold higher respectively than those for 2-oxoglutarate.

  将枯草芽孢杆菌的谷氨酸脱氢酶的Lys80，Gly82和Met101残基突变为一系列单个突变体。野生型酶对2-草酰戊二酸具有高度特异性，而G82K和M101S对草酰乙酸的特异性急剧增加，kcat值分别为3.45和5.68 s-1，分别比2-草酸高265倍和473倍。戊二酸。
• Production of L-amino acids by transamination
  申请人：Genetics Institute, Inc.

  公开号：US04518692A1

  公开（公告）日：1985-05-21

  A process is described for producing alpha amino acids or derivatives thereof. The process comprises reacting an alpha-keto acid with L-aspartic acid in the presence of transaminase enzyme to produce (1) an alpha amino acid corresponding to said alpha-keto acid and (2) oxaloacetate; and decarboxylating said oxaloacetate.

  描述了一种生产α-氨基酸或其衍生物的过程。该过程包括在转氨酶酶的存在下，将α-酮酸与L-天冬氨酸反应，产生（1）与所述α-酮酸相对应的α-氨基酸和（2）草酰乙酸；并脱羧化所述草酰乙酸。
• Method and composition for supplementing vitamin B6 where the PN-PLP
  申请人：Vesta Medicines (pty) Ltd.

  公开号：US05254572A1

  公开（公告）日：1993-10-19

  Treatment or prophylaxis of depressed or inadequate intracellular pyridoxal phosphate levels in a human or animal patient resulting from a condition, wherein the pyridoxihe (PN)--pyridoxal phosphate (PLP) pathway is disturbed or insufficient, either by chemical factors as occur in physiological shock myocardial, infarction, release of polyamines or toxins by cell death or microbes, vitamin B6 antagonistic drugs; or by enzymatic insufficiencies inherent in the cells of a patient caused by genetic lack of oxidase or genetic oxidase polymorphism; cellular immaturity of premature infants; in conditions involving anemia, destruction of erythrocytes (e.g. malaria, biliary fever). The deficiencies are counteracted by the administration of pyridoxal or a precursor of pyridoxal which in vivo, once it has entered the bloodstream, is rapidly converted into pyridoxal without the intervention of oxidase or oxygen, optionally and preferably without the intervention of kinase.

  治疗或预防由于某种情况导致的人类或动物患者细胞内吡哆醛磷酸水平降低的情况，其中吡哆醇（PN）-吡哆醛磷酸（PLP）途径受到干扰或不足，这种情况可能是由化学因素引起的，如生理性休克心肌梗死、细胞死亡或微生物释放的多胺或毒素、维生素B6拮抗药物；或由患者细胞内固有的酶不足引起的，可能是由于氧化酶的遗传缺乏或基因氧化酶多态性引起的；早产儿的细胞不成熟；在涉及贫血、红细胞破坏（如疟疾、胆热病）的情况下。这些不足可以通过给予吡哆醛或吡哆醇的前体来抵消，一旦进入血液循环，前体会迅速转化为吡哆醛，而不需要氧化酶或氧气的介入，可选择并且最好不需要激酶的介入。
• Racemic conversion of using a transaminase
  申请人：——

  公开号：US05316943A1

  公开（公告）日：1994-05-31

  Recombinant cells and an improved method utilizing them is disclosed for the preparation of an optically pure, L-amino acid from its D and D,L isomer forms. The process utilizes cell cultures that possess high aminotransferase activity that exhibits moderate selectivity in the conversion of D and L amino acids to their respective 2-keto acids as well as absolute stereospecificity in the conversion of the 2-keto acids to the L isomer alone.

  本发明揭示了利用重组细胞和改进的方法制备光学纯的L-氨基酸，该方法可以从其D和D，L异构体形式中制备。该过程利用具有高氨基转移酶活性的细胞培养物，该酶在将D和L氨基酸转化为相应的2-酮酸时表现出适度的选择性，而在将2-酮酸转化为L异构体时表现出绝对的立体选择性。
• Method and test kit for detecting an aflatoxin B.sub.1 and G.sub.1 using
  申请人：Neogen Corporation

  公开号：US04835100A1

  公开（公告）日：1989-05-30

  Novel monoclonal antibodies to an aflatoxin B.sub.1 and G.sub.1 in a test kit and used in a method of testing are described. The method for producing the monoclonal antibodies uses repeated administration of aflatoxin B.sub.1 or the related analog compound as a 1-position polypeptide to a murine and production of a hybridoma to generate the novel monoclonal antibodies. The novel antibodies have limited cross-reactivity to aflatoxins B.sub.2, G.sub.2 and M.sub.1. Aflatoxin B.sub.1 or aflatoxin G.sub.1 are detected in foods and the like using the test kit and method.

  本文介绍了一种用于测试的测试工具和方法，其中包括用于检测黄曲霉毒素B.sub.1和G.sub.1的新型单克隆抗体。制备单克隆抗体的方法是将黄曲霉毒素B.sub.1或相关的类似化合物反复注射到小鼠中作为1位多肽，然后制备杂交瘤以生成新型单克隆抗体。这些新型抗体对黄曲霉毒素B.sub.2、G.sub.2和M.sub.1的交叉反应有限。使用测试工具和方法可以检测食品等中的黄曲霉毒素B.sub.1或黄曲霉毒素G.sub.1。