Super silyl-based stable protecting groups for both the C- and N-terminals of peptides: applied as effective hydrophobic tags in liquid-phase peptide synthesis
作者:An Wu、Hisashi Yamamoto
DOI:10.1039/d3sc01239e
日期:——
Simple silyl groups have positive effects when incorporated in the tags due to their hydrophobic properties. Super silyl groups contain several simple silyl groups and play an important role in modern aldol reactions. In view of the unique structural architecture and hydrophobic properties of the super silyl groups, herein, two new types of stable super silyl-based groups (tris(trihexylsilyl)silyl group
标签辅助液相肽合成 (LPPS) 是药物发现中肽合成的重要过程之一。由于其疏水性,简单的甲硅烷基在结合到标签中时具有积极作用。超级甲硅烷基包含几个简单的甲硅烷基,在现代羟醛反应中起着重要作用。鉴于超级甲硅烷基独特的结构结构和疏水性,本文开发了两种新型稳定的超级甲硅烷基基团(三(三己基甲硅烷基)甲硅烷基和炔丙基超级甲硅烷基)作为疏水标签以增加溶解度在有机溶剂中和 LPPS 期间肽的反应性。三(三己基甲硅烷基)甲硅烷基可以酯形式安装在肽的C端和氨基甲酸酯形式的N端用于肽合成,并且与氢化条件(Cbz化学)和Fmoc-脱保护条件(Fmoc化学)相容). 炔丙基超级甲硅烷基是耐酸的,与 Boc 化学相容。这两个标签是互补的。这些标签的准备比以前报告的标签需要更少的步骤。使用这两种类型的超级甲硅烷基标签,通过不同的策略成功合成了 Nelipepimut-S。这些标签的准备比以前报告的标签需要更少的步骤