Inosin 5'-phosphorothioat | 21914-75-0

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: Inosin 5'-phosphorothioat
• 英文别名: Inosin-5'-phosphorthionat；P-thio-[5']inosinic acid；5'-Deoxy-5'-thioinosine 5'-monophosphate；9-[(2R,3R,4S,5R)-5-(dihydroxyphosphinothioyloxymethyl)-3,4-dihydroxyoxolan-2-yl]-1H-purin-6-one
• CAS: 21914-75-0
• 化学式: C₁₀H₁₃N₄O₇PS
• 分子量: 364.276
• InChiKey: GEIYZNFNTVEVJJ-KQYNXXCUSA-N

物化性质

• 密度：
  2.25±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -1.2
• 重原子数：
  23
• 可旋转键数：
  4
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  191
• 氢给体数：
  5
• 氢受体数：
  10

反应信息

• 作为反应物：
  描述：

  Inosin 5'-phosphorothioat 在 ligase 、 氯磷酸二苯酯 作用下， 生成
  参考文献：
  名称：

  On the mechanism of T4 RNA ligase

  摘要：

  DOI：

  10.1021/ja00393a047

文献信息

• Use of pimecrolimus for selective immunomodulation
  申请人：Novartis AG

  公开号：EP1649856A2

  公开（公告）日：2006-04-26

  The invention relates to methods to produce selective immunomodulation in epithelial tissues to treat epithelial inflammatory disorders. This invention provides methods for the use of pimecrolimus to treat epithelial inflammatory disorders including asthma and inflammatory bowel disease while minimizing systemic side effects.

  本发明涉及在上皮组织中产生选择性免疫调节以治疗上皮炎症性疾病的方法。本发明提供了使用吡美莫司治疗上皮炎症性疾病（包括哮喘和炎症性肠病）的方法，同时将全身副作用降至最低。
• Screening assay for the identification of agents which alter expression of PTH-rP
  申请人：Osteo Screen

  公开号：US20020061509A1

  公开（公告）日：2002-05-23

  A cell-based assay technique for identifying and evaluating chemical compounds and agents which affect the production of PTH-rP in mammalian cells and other cell types is set forth. Specifically, tumor cell lines are transformed with an expression vector comprising a DNA sequence encoding a promoter region of PTH-rP operatively linked to a reporter gene encoding an assayable product and cultured under conditions which permit expression of the assayable product. Chemical agents and factors can then be identified by their ability to modulate the expression of the reporter gene, thereby affecting the production of the assayable product. Such agents are then tested for inhibitory effects on tumor cell growth and for stimulatory effects on bone formation and repair.

  本发明提出了一种基于细胞的检测技术，用于鉴定和评估影响哺乳动物细胞和其他细胞类型中 PTH-rP 生成的化合物和制剂。具体来说，用一种表达载体转化肿瘤细胞系，该载体包含一个 DNA 序列，编码 PTH-rP 的启动子区域，该序列与编码可检测产物的报告基因操作性连接，并在允许可检测产物表达的条件下进行培养。然后，可以通过化学制剂和因子调节报告基因的表达从而影响可检测产物的产生的能力来确定这些制剂和因子。然后再检测这些制剂对肿瘤细胞生长的抑制作用以及对骨形成和修复的刺激作用。
• Methods for selective immunomodulation
  申请人：——

  公开号：US20030119797A1

  公开（公告）日：2003-06-26

  This invention relates to methods to produce selective immunomodulation in epithelial tissues to treat epithelial inflammatory disorders. This invention provides methods for the use of pimecrolimus to treat epithelial inflammatory disorders, including asthma and inflammatory bowel disease while minimizing systemic side effects.

  本发明涉及在上皮组织中产生选择性免疫调节以治疗上皮炎症性疾病的方法。本发明提供了使用吡美莫司治疗上皮炎症性疾病（包括哮喘和炎症性肠病）的方法，同时将全身副作用降至最低。
• Preservation of eukaryotic cells
  申请人：Hand C. Steven

  公开号：US20050084481A1

  公开（公告）日：2005-04-21

  A method is disclosed for reliably stabilizing eukaryotic cells that express the P2X 7 receptor channel, particularly mammalian and other vertebrate cells, including human cells, for example mammalian macrophages, or hematopoietic stem cells, in order to introduce otherwise membrane-impermeable compounds that are helpful for stabilizing the cells during drying, chilling, freezing, freeze-drying, or cryopreservation. The cells are exposed to extracellular ATP in concentration sufficient to open pores in the plasma membrane. One or more otherwise membrane-impermeable compounds that aid the survivorship of cells are then introduced, for example, trehalose, and after a brief time the pores are closed—for example, by adding divalent cations, or by diluting the extracellular solution. Once the trehalose or other stabilizing compound has been introduced, the cells may be stably preserved. By taking advantage of an endogenous mammalian receptor and ATP, no antigenic compounds need be introduced.

  本研究公开了一种方法，用于可靠地稳定表达 P2X 7 受体通道的真核细胞，特别是哺乳动物和其他脊椎动物细胞，包括人类细胞，例如哺乳动物巨噬细胞或造血干细胞，以便在干燥、冷冻、冷藏、冷冻干燥或低温保存过程中引入有助于稳定细胞的其他膜渗透性化合物。细胞暴露于浓度足以打开质膜孔的细胞外 ATP。然后引入一种或多种有助于细胞存活的膜不透性化合物，例如曲哈洛糖，经过短暂的时间后，孔隙被关闭--例如通过添加二价阳离子或稀释细胞外溶液。一旦引入树胶糖或其他稳定化合物，细胞就可以稳定地保存下来。利用哺乳动物的内源性受体和 ATP，无需引入抗原化合物。
• METHODS FOR SELECTIVE IMMUNOMODULATION USING PIMECROLIMUS
  申请人：Novartis AG

  公开号：EP1389108A2

  公开（公告）日：2004-02-18