2-Furoyl-CoA | 22342-70-7

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 英文名称: 2-Furoyl-CoA
• 英文别名: S-[2-[3-[[(2R)-4-[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoyl]amino]propanoylamino]ethyl] furan-2-carbothioate
• CAS: 22342-70-7
• 化学式: C₂₆H₃₈N₇O₁₈P₃S
• 分子量: 861.611
• InChiKey: IIKOXWXMCPEVLL-DJVIHCHSSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -4.6
• 重原子数：
  55
• 可旋转键数：
  21
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.54
• 拓扑面积：
  402
• 氢给体数：
  9
• 氢受体数：
  23

反应信息

• 作为反应物：
  描述：

  7,13-O,O-diacetyl-2-O-debenzoylbaccatin III 、 2-Furoyl-CoA 在 mutant 2-O-debenzoylbaccatin III 2-O-benzoyltransferase, containing point mutations: Q₁₉P and N₂₃K 作用下， 生成 7,13-O,O-diacetyl-2-O-debenzoyl-2-O-(2-furancarbonyl)baccatin III
  参考文献：
  名称：

  Point Mutations (Q19P and N23K) Increase the Operational Solubility of a 2α-O-Benzoyltransferase that Conveys Various Acyl Groups from CoA to a Taxane Acceptor

  摘要：

  Two site-directed Mutations within the wild-type 2-O-benzoyltransferase (tbt) cDNA, from Taxus cuspidata plants, yielded air encoded protein containing replacement amino acids at Q19P and N23K that trial) to a solvent-exposed loop region. The likely significant changes in the biophysical properties invoked by these mutations Caused the overexpressed, modified TBT (mTBT) to partition into the Soluble enzyme fraction about 5-fold greater than the wild-type enzyme. Sufficient protein could now be acquired to examine the scope of the substrate specificity of mTBT by incubation with 7,13-O,O-diacetyl-2-O-debenzoylbaceatin III that was mixed individually with various substituted benzoyls, alkanoyls, and (E)-butenoyl CoA donors. The mTBT catalyzed the conversion of each 7,13-O,O-diacetyl-2-O-debenzoylbaccatin III to several 7,13-O,O-diacetyl-2-O-acyl-2-O-debenzoylbaccatin III analogues. The relative catalytic efficiency of mTBT with the 7,13-O,O-diacetyl-2-O-debenzoyl surrogate Substrate and heterole carbonyl CoA substrates was slightly greater than with the natural aroyl substrate benzoyl CoA, While substituted benzoyl CoA thioesters were less productive. Short-chain hydrocarbon carbonyl and cyclohexanoyl CoA thioesters were also productive, where C-4 Substrates were transferred by mTBT with similar to 10- to 17-fold greater catalytic efficiency compared to the transfer of benzoyl. The described broad specificity of mTBT suggests (flat a plethora of 2-O-acyl variants of the antimitotic paclitaxel can be assembled through biocatalytic sequences.

  DOI：

  10.1021/np900524d
• 作为产物：
  描述：

  糠酸（呋喃甲酸） 在 potassium carbonate 、 N,N'-二环己基碳二亚胺 作用下， 以 1,4-二氧六环 、 水 、 N,N-二甲基甲酰胺 为溶剂， 反应 24.0h， 生成 2-Furoyl-CoA
  参考文献：
  名称：

  Insights into β-ketoacyl-chain recognition for β-ketoacyl-ACP utilizing AHL synthases

  摘要：

  报道了用于两种3-氧代酰-ACP利用类群信号合成酶的β-酮酰-ACP模拟物的设计、合成和动力学评价。

  DOI：

  10.1039/c8cc04532a

文献信息

• Novel microorganism and its use in lignocellulose detoxification
  申请人：Shell Internationale Research Maatschappij B.V.

  公开号：EP2295534A1

  公开（公告）日：2011-03-16

  The present invention relates to an isolated microorganism which is Cupriavidus basilensis strain HMF14 Deposit number DSM 22875, and its use in a process for the in-situ detoxification of lignocelluloses hydrolysate.

  本发明涉及一种分离出的微生物（Cupriavidus basilensis 菌株 HMF14 保藏号 DSM 22875）及其在木质纤维素水解物原位解毒工艺中的应用。
• Novel microorganism and its use in lignocellulose detoxifixcation
  申请人：Shell Internationale Research Maatschappij B.V.

  公开号：EP2308959A1

  公开（公告）日：2011-04-13

  The present invention relates to an isolated microorganism which is Cupriavidus basilensis strain HMF14 Deposit number DSM 22875, and its use in a process for the in-situ detoxification of lignocelluloses hydrolysate

  本发明涉及一种分离出的微生物（Cupriavidus basilensis 菌株 HMF14 保藏号 DSM 22875）及其在木质纤维素水解物原位解毒工艺中的应用。
• WATER SOLUBLE COMPOSITIONS INCORPORATING ENZYMES, AND METHOD OF MAKING SAME
  申请人：Monosol, LLC

  公开号：EP3354716A1

  公开（公告）日：2018-08-01

  Disclosed herein are water soluble compositions, such as films, including a mixture of a first water-soluble resin, an enzyme, and an enzyme stabilizer which comprises a functional substrate for the enzyme, methods of making such compositions, and methods of using such compositions, e.g. to make packets containing functional ingredients. The enzymes can include proteases and mixtures of proteases with other enzymes, and the compositions provide good retention of enzyme function following film processing and storage.

  本文公开了水溶性组合物，例如薄膜，包括第一水溶性树脂、酶和酶稳定剂的混合物，其中酶稳定剂包括酶的功能底物，还公开了制造这种组合物的方法，以及使用这种组合物的方法，例如制造含有功能成分的包装。酶可以包括蛋白酶和蛋白酶与其他酶的混合物，这种组合物在薄膜加工和储存后能很好地保持酶的功能。
• Methods for conducting assays for enzyme activity on protein microarrays
  申请人：Zhou X. Fang

  公开号：US20050118665A1

  公开（公告）日：2005-06-02

  The present invention relates to methods of conducting assays for enzymatic activity on microarrays useful for the large-scale study of protein function, screening assays, and high-throughput analysis of enzymatic reactions. The invention relates to methods of using protein chips to assay the presence, amount, activity and/or function of enzymes present in a protein sample on a protein chip. In particular, the methods of the invention relate to conducting enzymatic assays using a microarray wherein a protein and a substance are immobilized on the surface of a solid support and wherein the protein and the substance are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the substance and the protein. The invention also relates to microarrays that have an enzyme and a substrate immobilized on their surface wherein the enzyme and the substrate are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the enzyme and the substrate.

  本发明涉及在微阵列上进行酶活性测定的方法，这种方法可用于蛋白质功能的大规模研究、筛选测定和酶反应的高通量分析。本发明涉及使用蛋白质芯片检测蛋白质芯片上蛋白质样品中酶的存在、数量、活性和/或功能的方法。特别是，本发明的方法涉及使用芯片进行酶测定，其中蛋白质和物质被固定在固体支持物表面，蛋白质和物质相互靠近，足以使物质和蛋白质之间发生酶反应。本发明还涉及在其表面固定有酶和底物的微阵列，其中酶和底物相互靠近，足以使酶和底物之间发生酶反应。
• NOVEL MICROORGANISM AND ITS USE IN LIGNOCELLULOSE DETOXIFICATION
  申请人：KOOPMAN Frank Wouter

  公开号：US20110086395A1

  公开（公告）日：2011-04-14

  An isolated microorganism is provided which is Cupriavidus basilensis strain HMF14 Deposit number DSM 22875, and its use in a process for the in-situ detoxification of lignocelluloses hydrolysate.