N-succinimidyl-4-hydroxy-3-methoxybenzoate | 70120-42-2

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: N-succinimidyl-4-hydroxy-3-methoxybenzoate
• 英文别名: Vanillinsaeure-N-hydroxysuccinimidester；(2,5-Dioxopyrrolidin-1-yl) 4-hydroxy-3-methoxybenzoate
• CAS: 70120-42-2
• 化学式: C₁₂H₁₁NO₆
• 分子量: 265.222
• InChiKey: WWQQEXCXNQDYFN-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.9
• 重原子数：
  19
• 可旋转键数：
  4
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  93.1
• 氢给体数：
  1
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  N-羟基丁二酰亚胺 、 香草酸 在 N,N'-二环己基碳二亚胺 作用下， 以 二氯甲烷 、 乙酸乙酯 、 N,N-二甲基甲酰胺 为溶剂， 以70%的产率得到N-succinimidyl-4-hydroxy-3-methoxybenzoate
  参考文献：
  名称：

  Pyridylthiourea-grafted polyethylenimine offers an effective assistance to siRNA-mediated gene silencing in vitro and in vivo

  摘要：

  Success of synthetic interfering nucleic acids (siRNAs)-based therapy relies almost exclusively on effective, safe and preferably nanometric delivery systems which can be easily prepared, even at high concentrations. We prepared by chemical synthesis various self-assembling polymers to entrap siRNAs into stable polyplexes outside cells but with a disassembly potential upon sensing endosomal acidity. Our results revealed that pyridylthiourea-grafted polyethylenimine (pi PEI) followed the above-mentioned principles. It led to above 90% siRNA-mediated gene silencing in vitro on U87 cells at 10 nM siRNA concentration and did not have a hemolytic activity. Assembly of siRNA/pi PEI. at high concentration was then studied and 4.5% glucose solution, pH 6.0, yielded stable colloidal solutions with sizes slightly below 100 nm for several hours. A single injection of these concentrated siRNA polyplexes into luciferase-expressing human glioblastoma tumors, which were subcutaneously xenografted into nude mice, led to a significant 30% siRNA-mediated luciferase gene silencing 4 days post-injection. Our results altogether substantiate the potential of self-assembling cationic polymers with a pH-sensitive disassembly switch for siRNA delivery in vitro and also in vivo experiments. (C) 2011 Elsevier B. V. All rights reserved.

  DOI：

  10.1016/j.jconrel.2011.10.007

文献信息

• Production of vanillin
  申请人：——

  公开号：US20010014467A1

  公开（公告）日：2001-08-16

  A method of producing vanillin comprising the steps of: (1) providing trans-ferulic acid or a salt thereof; and (2) providing trans-ferulate: CoASH ligase activity (enzyme activity I), trans-feruloyl ScoA hydratase activity (enzyme activity II), and 4-hydroxy-3-methoxyphenyl-&bgr;-hydroxy-propionyl SCoA (HMPHP SCoA) cleavage activity (enzyme activity III). Conveniently the enzymes are provided by Pseudomonas fluorescens Fe3 or a mutant or derivative thereof. Polypeptides with enzymes activities II and III and polynucleotides encoding said polypeptides. Use of said polypeptides or said polynucleotides in a method for producing vanillin.

  一种生产香草醛的方法，包括以下步骤：（1）提供反式阿魏酸或其盐；（2）提供反式阿魏酸：辅酶A硫酯酰化酶活性（酶活性I）、反式阿魏酰辅酶A水合酶活性（酶活性II）和4-羟基-3-甲氧基苯基-β-羟基-丙酰辅酶A（HMPHP SCoA）裂解酶活性（酶活性III）。方便起见，酶可以由铁蓝单胞菌Fe3或其突变体或衍生物提供。具有酶活性II和III的多肽以及编码该多肽的多核苷酸。使用所述多肽或所述多核苷酸的方法生产香草醛。
• POLYMERS FOR DELIVERING MOLECULES OF INTEREST
  申请人：Zuber Guy

  公开号：US20130096177A1

  公开（公告）日：2013-04-18

  The present invention relates to a new class of cationic polymers that self-assemble with a pH-sensitive dissolution switch, and their uses to deliver molecules of interest to a cell. The present invention also relates to compositions comprising said cationic polymers non-covalently associated with a molecule of interest, in particular with a siRNA.

  本发明涉及一种新型阳离子聚合物，它们可以自组装并带有pH敏感的溶解开关，并且它们的用途是将感兴趣的分子传递到细胞中。本发明还涉及包含所述阳离子聚合物的组合物，该组合物与感兴趣的分子非共价地结合，特别是与siRNA结合。
• Production of p-hydroxybenzoic acid
  申请人：——

  公开号：US20030167511A1

  公开（公告）日：2003-09-04

  One aspect of the present invention relates to a transgenic plant which, by presence of a transgene, is able to produce p-hydroxybenzoic acid or a &bgr;-D-glycoside or &bgr;-D-glucose ester thereof. A method is also disclosed for producing p-hydroxybenzoic acid or a &bgr;-D-glycoside or &bgr;-D-glucose ester thereof using a transgenic plant of the present invention.

  本发明的一个方面涉及一种转基因植物，通过转基因的存在，该植物能够生产对羟基苯甲酸或其&bgr;-D-糖苷或&bgr;-D-葡萄糖酯。还公开了一种利用本发明的转基因植物生产对羟基苯甲酸或其&bgr;-D-糖苷或&bgr;-D-葡萄糖酯的方法。
• PRODUCTION OF VANILLIN
  申请人：INSTITUTE OF FOOD RESEARCH

  公开号：EP0904396A2

  公开（公告）日：1999-03-31
• US5290937A
  申请人：——

  公开号：US5290937A

  公开（公告）日：1994-03-01