Ν,Ν-diisopropylamine tetrazonide | 4772-43-4

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 唑类
• 英文名称: Ν,Ν-diisopropylamine tetrazonide
• 英文别名: 1-Diisopropylamino-tetrazol；diisopropyl ammonium tetrazolide；diisopropyl-tetrazol-1-yl-amine；diisopropylaminotetrazole；N,N-diisopropylamine tetrazolide；N,N-diisopropylamine tetrazonide；diisopropylamine tetrazolide；tetrazole diisopropylamine；N,N-Di(propan-2-yl)-1H-tetrazol-1-amine；N,N-di(propan-2-yl)tetrazol-1-amine
• CAS: 4772-43-4
• 化学式: C₇H₁₅N₅
• 分子量: 169.23
• InChiKey: KOOSVDLGLFCZEH-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.7
• 重原子数：
  12
• 可旋转键数：
  3
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.86
• 拓扑面积：
  46.8
• 氢给体数：
  0
• 氢受体数：
  4

文献信息

• NUCLEIC ACID MONOMERS WITH 2'-CHEMICAL MOIETIES
  申请人：Laikhter Andrei

  公开号：US20070218490A1

  公开（公告）日：2007-09-20

  The invention provides nucleic acid monomers with a 2′-modification that are useful for the incorporation of dyes or blocking groups. The monomers can be incorporated on the 3′-end of a dual labeled probe to inhibit PCR polymerase extension during PCR. The polymerase is inhibited from extending the probe at the 3′-hydroxyl group when the monomer is present; there is no need to add a chemical moiety to the 3′-hydroxyl or remove the 3′-hydroxyl. The monomers can also be incorporated internally or at the 5′-end of the oligonucleotide. A detectable label, such as a fluorescent or quenching dye, can be incorporated on the 2′-position of such monomers.

  该发明提供了具有2'-修饰的核酸单体，可用于染料或阻断基团的引入。这些单体可以被合并到双标记探针的3'-末端，以在PCR过程中抑制PCR聚合酶的延伸。当单体存在时，聚合酶被抑制在3'-羟基团上延伸探针；无需向3'-羟基团添加化学基团或去除3'-羟基团。这些单体也可以在寡核苷酸的内部或5'-末端进行合并。可以在这些单体的2'-位置上合并可检测的标记，如荧光染料或猝灭染料。
• Labeled oligonucleotides, methods for making same, and compounds useful therefor
  申请人：——

  公开号：US20030208061A1

  公开（公告）日：2003-11-06

  Selectively functionalized oligonucleotides, methods for making same, and compounds useful therefor are disclosed. The oligonucleotides can be selectively functionalized with a first conjugate group at the 3′-terminial position and optionally functionalized with a second conjugate group at the 5′-terminal position and/or one or more internucleotides. Alternatively, the oligonucleotides can be selectively functionalized with a first conjugate group at the 5′-terminal position and optionally functionalized with a second conjugate group at one or more internucleotides. In yet another embodiment, the oligonucleotides can be functionalized with a first conjugate group at one or more internucleotides and with a second conjugate group at one or more different internucleotides.

  选择性功能化寡核苷酸，制备方法以及用于此目的的化合物已被披露。这些寡核苷酸可以在3'-末端位置选择性地与第一共轭基团功能化，并可选择性地在5'-末端位置和/或一个或多个核苷酸之间与第二共轭基团功能化。另外，这些寡核苷酸可以在5'-末端位置选择性地与第一共轭基团功能化，并可选择性地在一个或多个核苷酸之间与第二共轭基团功能化。在另一实施方式中，这些寡核苷酸可以在一个或多个核苷酸之间与第一共轭基团功能化，并在一个或多个不同的核苷酸之间与第二共轭基团功能化。
• [EN] PHOSPHOLIPID ANALOGUES<br/>[FR] ANALOGUES DE PHOSPHOLIPIDES
  申请人：KARL FRANZENS UNIV GRAZ

  公开号：WO2019025324A1

  公开（公告）日：2019-02-07

  The present invention relates to a compound having a structure according to formula (I) or a salt thereof, wherein : R1 is a branched or linear alkyl, acyl, alkenyl, hydroxyalkyl, hydroxyalkenyl, or hydroxy-alkinyl residue comprising 6 to 30 carbon atoms, R2 is selected from the group consisting of : a) an alkyl residue comprising 1 to 10 carbon atoms and comprising a terminal or cyclic quaternary ammonium, b) an alkyl residue comprising 1 to 10 carbon atoms and comprising a terminal amino group and/or 1 to 3 hydroxyl groups, c) an amino acid residue, d) a five or six carbon sugar and e) H, R3 is a residue selected from the group consisting of an alkyl, alkenyl, prostanyl-dialkyl, furanyl-dialkyl, cyclobutyl-dialkyl, cycloalkyl and aryl group of 2 to 40 carbon atoms length and comprising at least one keto group, epoxy group, aldehyde group, peroxy group, hydroperoxy group, hydroxyl group or a free carboxyl group, L1 is selected from the group consisting of O, NH, S, CH2, (aa), (bb), (cc), (dd), (ee), (ff), (gg), (hh), (ii), (jj), (kk), (ll), (mm), or (nn), L2 is an amide group or an ether group, X and Z are independently O, S or CH2, and Y is selected from the group consisting of O and S.

  本发明涉及具有如下式(I)的结构或其盐的化合物，其中：R1为支链或直链烷基、酰基、烯基、羟基烷基、羟基烯基或含有6至30个碳原子的羟基-炔基残基，R2选自以下组：a）含有1至10个碳原子且含有末端或环状季铵基的烷基残基，b）含有1至10个碳原子且含有末端氨基和/或1至3个羟基的烷基残基，c）氨基酸残基，d）五元或六元碳糖和e）H，R3为选自以下组的残基：长度为2至40个碳原子的烷基、烯基、前列腺烷基二烷基、呋喃烷基二烷基、环丁基二烷基、环烷基和芳基，并且至少含有一个酮基、环氧基、醛基、过氧基、过氧羟基、羟基或自由羧基，L1选自以下组：O、NH、S、CH2、（aa）、（bb）、（cc）、（dd）、（ee）、（ff）、（gg）、（hh）、（ii）、（jj）、（kk）、（ll）、（mm）或（nn），L2为酰胺基或醚基，X和Z独立地为O、S或CH2，Y选自以下组：O和S。
• FUNCTIONALIZED 3-ALKYNYL PYRAZOLOPYRIMIDINE ANALOGUES AS UNIVERSAL BASES AND METHODS OF USE
  申请人：Vorobiev Alexei

  公开号：US20130261014A1

  公开（公告）日：2013-10-03

  3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.

  提供了3-炔基肌苷类似物及其作为通用碱基的用途。这些肌苷类似物可以被合并到核酸引物和探针中。它们不会显著破坏核酸双链。因此，包含这些肌苷类似物的新型核酸引物和探针可以用于各种方法。这些类似物意外地作为通用碱基发挥良好作用。它们不仅比嘌呤对A、C、T和G稳定双链更多，而且在引物中被聚合酶识别，从而实现高效扩增。
• Novel phosphate and thiophosphate protecting groups
  申请人：——

  公开号：US20040082774A1

  公开（公告）日：2004-04-29

  Novel P(III) bisamidite reagents as phosphorus protecting groups, nucleoside phosphoramidite intermediates, and synthetic processes for making the same are disclosed. Furthermore, oligomeric compounds are prepared through the protection of one or more internucleosidic phosphorus functionalities, preferably followed by oxidation and cleavage of the protecting groups to provide oligonucleotides. Methods for preparing oligoribonucleotides are also disclosed.

  本发明揭示了Novel P(III)双酰胺试剂作为磷保护基，核苷酸磷酰胺中间体和制备它们的合成过程。此外，通过保护一个或多个核苷酸之间的磷功能基，最好是随后进行氧化和去保护基，以提供寡核苷酸，制备寡聚化合物。本发明还揭示了制备寡核糖核酸的方法。