2-amino-9-[(2R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-purin-6-one

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: 2-amino-9-[(2R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-purin-6-one
• 化学式: C₁₀H₁₃N₅O₅
• 分子量: 283.24
• InChiKey: NYHBQMYGNKIUIF-BMWIRTBBSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -2.7
• 重原子数：
  20
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  155
• 氢给体数：
  5
• 氢受体数：
  6

文献信息

• Method for colorimetric determination of cPNMP specific enzyme activity via coupled enzymatic reactions
  申请人：Semmelweis Egyetem

  公开号：EP2514832A1

  公开（公告）日：2012-10-24

  The invention relates to a method for determination of phosphodiesterase enzyme (PDE) activity or the inhibitory activity of an inhibitor candidate molecule by colorimetric assay. The invention primarily relates to the coupled usage of enzymes and enzyme specific substrates for the colorimetric determination of the enzyme activity in the enzyme catalyzed conversion of cyclic purine nucleoside monophosphate (cPNMP) to opened PNMP (e.g. cGMP to GMP). The invention is based on the discovery that four or five enzymes can be coupled in a one pot reaction to provide a final colored mixture that is suitable for qualitative or quantitative analysis.

  本发明涉及一种通过比色法测定磷酸二酯酶（PDE）活性或候选抑制剂分子抑制活性的方法。本发明主要涉及酶和酶特异性底物的联用，用于比色测定酶催化环状嘌呤核苷单磷酸（cPNMP）转化为开放型 PNMP（如 cGMP 转化为 GMP）过程中的酶活性。本发明的基础是发现四种或五种酶可以在一锅反应中耦合，以提供适合定性或定量分析的最终彩色混合物。