申请人:DUPONT NUTRITION BIOSCIENCES APS
公开号:US10612014B2
公开(公告)日:2020-04-07
The present invention relates to a method for solubilizing arabinoxylan-containing material (particularly insoluble arabinoxylan-containing material), comprising admixing a xylan-containing material with a xylanase comprising a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11 or SEQ ID No. 15, or a variant, homolog, fragment or derivative thereof having at least 75% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1 or SEQ ID No. 9 or SEQ ID No. 10 or SEQ ID No. 11 or SEQ ID No. 15; or a polypeptide sequence which comprises SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11 or SEQ ID No. 15 with a conservative substitution of at least one of the amino acids; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 17 or SEQ ID No. 18, or a nucleotide sequence which can hybridize to SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 17 or SEQ ID No. 18 under high stringency conditions, or a nucleotide sequence which has at least 75% identity with SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 17 or SEQ ID No. 18, or a nucleotide sequence which differs from SEQ ID No. 6 or SEQ ID No. 5 or SEQ ID No. 4 or SEQ ID No. 12 or SEQ ID No. 13 or SEQ ID No. 14 or SEQ ID No. 16 or SEQ ID No. 17 or SEQ ID No. 18 due to the degeneracy of the genetic code, or a xylanase obtainable (or obtained) from Fusarium verticilloides. The present invention also relates to a novel xylanase comprising (or consisting of) a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1, or a variant, homolog, fragment or derivative thereof having at least 99% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5 or SEQ ID No. 4, or a nucleotide sequence which can hybridize to SEQ ID No. 4 or SEQ ID No. 5 under high stringency conditions, or a nucleotide sequence which has at least 97.7% identity (preferably 98% identity) with SEQ ID No. 6, SEQ ID No. 5 or SEQ ID No. 4. The present invention yet further relates to methods relating to feedstuffs, malting and brewing, processing of grain-based materials such as during the production of bioethanol or biochemical (e.g. bio-based isopropanol), or wheat gluten-starch separation processes and the like.
本发明涉及一种增溶含阿拉伯
木聚糖材料(特别是不溶性含阿拉伯
木聚糖材料)的方法,包括将含
木聚糖材料与
木聚糖酶混合,
木聚糖酶包括本文所示的
多肽序列
SEQ ID No.3、
SEQ ID No.2、
SEQ ID No.1、
SEQ ID No.9、
SEQ ID No.10、
SEQ ID No.11或
SEQ ID No.15,或其与
SEQ ID No.3 or
SEQ ID No.9、
SEQ ID No.10、
SEQ ID No.11 或
SEQ ID No.15,其中至少有一个
氨基酸被保守取代的
多肽序列;或由本文所示核苷酸序列
SEQ ID No.6、
SEQ ID No.5、
SEQ ID No.4、
SEQ ID No.12、
SEQ ID No.13、
SEQ ID No.14、
SEQ ID No.16、
SEQ ID No.17 或
SEQ ID No.4、
SEQ ID No.12、
SEQ ID No.13、
SEQ ID No.14、
SEQ ID No.16、
SEQ ID No.17 或
SEQ ID No.5、
SEQ ID No.4、
SEQ ID No.12、
SEQ ID No.13、
SEQ ID No.14、
SEQ ID No.16、
SEQ ID No.17 或
SEQ ID No.12或
SEQ ID No.13或
SEQ ID No.14或
SEQ ID No.16或
SEQ ID No.17或
SEQ ID No.18不同的核苷酸序列,或可从疣镰刀菌中获得(或得到)的
木聚糖酶。本发明还涉及一种新型
木聚糖酶,该酶包含(或由)本文所示的
SEQ ID No.3、
SEQ ID No.2 或
SEQ ID No.1 的
多肽序列,或与
SEQ ID No.3 或
SEQ ID No.2 或
SEQ ID No.1;或由本文所示的
SEQ ID No.6、
SEQ ID No.5或
SEQ ID No.4的核苷酸序列编码的
木聚糖酶,或在高严格条件下能与
SEQ ID No.4或
SEQ ID No.5杂交的核苷酸序列,或与
SEQ ID No.6、
SEQ ID No.5或
SEQ ID No.4具有至少97.7%同一性(优选98%同一性)的核苷酸序列。本发明还进一步涉及与饲料、麦芽加工和酿造、谷物材料加工(如
生物乙醇或
生物化学(如
生物基
异丙醇)生产过程中)或小麦面筋-淀粉分离过程等有关的方法。