Provided herein are methods of nucleic acid sequencing using nucleotides with labels attached to the phosphate group so that incorporation of such nucleotides into a primed template results in formation of a phospho-label. Treatment of the phospho-label with a phophatase generates a free label which can be detected in a variety of ways. The labels can include, e.g., chemiluminescent labels, chemiluminescent substrates and enzyme activators. Also provided are reagents such as nucleotides phospholinked to labels such as enzyme activators.
[EN] SEQUENCING NUCLEIC ACIDS BY ENZYME ACTIVATION<br/>[FR] SÉQUENÇAGE D'ACIDES NUCLÉIQUES PAR L'ACTIVATION D'ENZYME
申请人:CAERUS MOLECULAR DIAGNOSTICS
公开号:WO2014110287A1
公开(公告)日:2014-07-17
Provided herein are methods of nucleic acid sequencing using nucleotides with labels attached to the phosphate group so that incorporation of such nucleotides into a primed template results in formation of a phospho-label. Treatment of the phospho-label with a phophatase generates a free label which can be detected in a variety of ways. The labels can include, e.g., chemiluminescent labels, chemiluminescent substrates and enzyme activators. Also provided are reagents such as nucleotides phospholinked to labels such as enzyme activators.