2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: 2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride
• 化学式: C22H31Cl2N7O5
• 分子量: 544.4
• InChiKey: MKSVFGKWZLUTTO-OVXKXQBWSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.05
• 重原子数：
  36
• 可旋转键数：
  8
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.45
• 拓扑面积：
  161
• 氢给体数：
  6
• 氢受体数：
  10

文献信息

• System for screening cells for high expression of a protein of interest
  申请人：Applied Research Systems ARS Holding N.V.

  公开号：EP1760092A1

  公开（公告）日：2007-03-07

  This invention refers to industrial production of proteins. More particularly, the invention refers to a fusion protein as a novel chimeric selection marker comprising a peptide conferring resistance to an antibiotic, or a fragment, allelic variant, splice variant or mutein thereof, and at least one sequence comprising SEQ ID NO: 1, 2 or 3, preferably for producing a protein of interest (POI). The inventive chimeric selection marker exhibits: (i) a resistance to an antibiotic; and (ii) a fluorescence activity upon binding of a ligand to the sequence comprising SEQ ID NO: 1, 2 or 3. The invention further refers to nucleic acids encoding the inventive fusion protein and to expression vectors, comprising the inventive fusion protein and additionally the protein of interest (POI). Finally, uses of the inventive chimeric selection marker for screening cells for high expression of a protein of interest (POI) are disclosed.

  本发明涉及蛋白质的工业生产。更具体地说，本发明是指一种作为新型嵌合选择标记物的融合蛋白，它包含对抗生素具有抗性的多肽，或其片段、等位基因变体、剪接变体或静音蛋白，以及至少一个包含 SEQ ID NO：1、2 或 3 的序列，优选用于生产感兴趣的蛋白质（POI）。本发明的嵌合选择标记表现出：(i) 对抗生素的抗性；(ii) 配体与包含 SEQ ID NO：1、2 或 3 的序列结合时的荧光活性。本发明还涉及编码本发明融合蛋白的核酸和表达载体，表达载体包括本发明融合蛋白和感兴趣的蛋白（POI）。最后，本发明还公开了本发明嵌合选择标记的用途，用于筛选高表达相关蛋白（POI）的细胞。
• System for Screening Cells for High Expression of a Protein of Interest (Poi)
  申请人：Dupraz Philippe

  公开号：US20080286824A1

  公开（公告）日：2008-11-20

  This invention refers to industrial production of proteins. More particularly, the invention refers to a fusion protein as a novel chimeric selection marker comprising a peptide conferring resistance to an antibiotic, or a fragment, allelic variant, splice variant or mutein thereof, and at least one sequence comprising SEQ ID NO: 1, 2 or 3, preferably for producing a protein of interest (POI). The inventive chimeric selection marker exhibits: (i) a resistance to an antibiotic; and (ii) a fluorescence activity upon binding of a ligand to the sequence comprising SEQ ID NO: 1, 2 or 3. The invention further refers to nucleic acids encoding the inventive fusion protein and to expression vectors, comprising the inventive fusion protein and additionally the protein of interest (POI). Finally, uses of the inventive chimeric selection marker for screening cells for high expression of a protein of interest (POI) are disclosed.