LDS 751 dye | 181885-68-7

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 英文名称: LDS 751 dye
• 英文别名: 2-[(1E,3E)-4-[4-(dimethylamino)phenyl]buta-1,3-dienyl]-1-ethyl-N,N-dimethylquinolin-1-ium-6-amine;perchlorate
• CAS: 181885-68-7
• 化学式: C25H30ClN3O4
• 分子量: 472.0
• InChiKey: FGBAVQUHSKYMTC-UHFFFAOYSA-M

物化性质

• 溶解度：
  可溶于DMSO（轻微）、甲醇（非常轻微）

计算性质

• 辛醇/水分配系数(LogP)：
  0.25
• 重原子数：
  33
• 可旋转键数：
  6
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.24
• 拓扑面积：
  84.6
• 氢给体数：
  0
• 氢受体数：
  6

制备方法与用途

生物活性

LDS-751 是一种主要检测 DNA 的核酸染色剂。这种染色剂对 DNA 有很高的亲和力，结合后荧光增强，但其最大发射波长为 670nm。LDS-751 和 Thiazole orange 可用于区分红细胞、血小板、网织红细胞和有核细胞，并能在 488nm 处激发。

体外研究

LDS-751 允许通过染色来区分完整细胞与残留的红细胞碎片、血小板及受损的有核细胞。使用 LDS-751 染色后，完整的细胞其正向和侧向光散射信号可清晰地区分主要的白细胞群体，因为受损的有核细胞、红细胞、红细胞碎片以及血小板已被去除。

文献信息

• Imaging of protease activity in live cells using activity based probes
  申请人：Bogyo S. Matthew

  公开号：US20070036725A1

  公开（公告）日：2007-02-15

  Methods and materials for the imaging of cells containing active proteases such as cathepsin are disclosed. The present materials include activity based probes that bind to an enzyme and are subsequently cleaved. Cleavage results in a fluorescent signal due to removal of a quenching group which, when present on the probe causes altered or no fluorescence. The probes employ an acyloxymethyl ketone reactive group, one or more amino acids for determining specificity, a fluorophore and a quencher. The probes are cell permeable and may use, for example, a QSY7 (diarylyrhodamine) quencher and a BODIPY (bora-diaza-indecene) dye.

  揭示了用于成像含有活性蛋白酶（如半胱氨酸蛋白酶）的细胞的方法和材料。目前的材料包括结合到酶并随后被切割的活性基团探针。切割导致荧光信号的产生，因为去除了一个熄灭基团，当存在于探针上时，会导致改变或无荧光。这些探针使用酰氧甲基酮反应基团，一个或多个氨基酸用于确定特异性，一个荧光团和一个熄灭剂。这些探针具有细胞穿透性，例如，可以使用QSY7（二芳基罗丹明）熄灭剂和BODIPY（硼二氮杂环庚烯）染料。
• A method for carrying out a laboratory test of the in vitro integrity of haematic cells with no fixation and/or permeabilization, by DNA and general nucleic acid labeling and by flow cytometry reading
  申请人：Petraroli, Salvatore

  公开号：EP0731179A2

  公开（公告）日：1996-09-11

  A method for carrying out a laboratory test of apoptosis of haematic cells in vitro with no fixation/permeabilization procedures, said method comprising the seeding of purified leukocytes, prepared by any way, within a test tube; the dyeing of DNA and of the nucleic acids in general; cold washing in an isotonic solution; suspension in a solution suitable for performing the reading of results by flow cytofluorometry. Reproducible results have been obtained even though the cells, before dyeing the DNA and the nucleic acids in general, are fixed by means of solutions containing typically paraformaldehyde in order to inactivate any pathogens possibly present.

  一种实验室检测体外血细胞凋亡的方法，无需固定/过稳定程序，该方法包括将以任何方式制备的纯化白细胞播种到试管中；对 DNA 和一般核酸进行染色；在等渗溶液中进行冷洗涤；悬浮在适合用流式细胞荧光测定法读取结果的溶液中。即使在对 DNA 和核酸进行染色之前，用通常含有多聚甲醛的溶液对细胞进行固定，以灭活可能存在的病原体，也能获得可重复的结果。
• Detection system
  申请人：——

  公开号：US20040241679A1

  公开（公告）日：2004-12-02

  A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising: performing nucleic acid amplification on the sample in the presence of (a) a DNA duplex binding agent, (b) a nucleic acid polymerase and (c) a reagent comprising an amplification primer which can hybridise to said target sequence when in single stranded form and which is connected at its 5′ end to a probe which carries a label by way of a chemical linking group, said labelled probe being of a sequence which is similar to that of the said target nucleic acid sequence, such that it can hybridise to a complementary region in an amplification product, and wherein the label is able to absorb fluorescence from or donate fluorescent energy to the DNA duplex binding agent; and monitoring fluorescence of said sample.

  一种检测样品中是否存在目标核酸序列的方法，该方法包括在以下试剂存在的情况下对样品进行核酸扩增：(a) DNA 双链结合剂；(b) 核酸聚合酶；(c) 由扩增引物组成的试剂，扩增引物在单链形式时可与所述目标序列杂交，其 5′端通过化学连接基团与带有标记的探针相连、所述标记探针的序列与所述目标核酸序列的序列相似，因此可以与扩增产物中的互补区杂交，其中标签能够吸收 DNA 双链结合剂的荧光或将荧光能量捐赠给 DNA 双链结合剂；监测所述样品的荧光。
• Method for analysis of subpopulations of blood cells
  申请人：Becton, Dickinson and Company

  公开号：EP0193356B1

  公开（公告）日：1991-12-04
• NUCLEIC ACID DETECTION METHOD
  申请人：The Secretary of State DSTL

  公开号：EP1390542A2

  公开（公告）日：2004-02-25