A modified polypeptide having carbohydrate processing enzymatic activity is provided, said polypeptide comprising an amino acid sequence selected from: (a) the amino acid sequence of SEQ ID NO:2 comprising a mutation in at least one of W433, E432 and M439; (b) the amino acid sequence of an enzyme of glycosyl hydrolase family 1, comprising at least one mutation at an amino acid residue equivalent to W433, E432 or M439 of SEQ ID NO:2; and (c) a variant of (a) or (b) having carbohydrate processing enzymatic activity and comprising at least one amino acid mutation at a position equivalent to W433, E432 or M439 of SEQ ID NO:2.
提供一种具有
碳水化合物加工酶活性的改性
多肽,该
多肽包括以下
氨基酸序列之一:(a)
SEQ ID NO: 2的
氨基酸序列,其中至少有一个W433、E432和
M439突变;(b) 包括至少一个
氨基酸残基等同于
SEQ ID NO:2的W433、E432或
M439的糖基
水解酶家族1酶的
氨基酸序列;以及(c) 具有
碳水化合物加工酶活性的(a)或(b)的变体,其中包括至少一个
氨基酸突变位于等同于
SEQ ID NO:2的W433、E432或
M439的位置。