[EN] EMBEDDING MEDIUM OF BIOLOGICAL SAMPLE FOR ITS IMAGING BY LIGHT AND/OR ELECTRON MICROSCOPY<br/>[FR] MILIEU D'INCORPORATION D'ÉCHANTILLON BIOLOGIQUE POUR SON IMAGERIE PAR MICROSCOPIE OPTIQUE ET/OU ÉLECTRONIQUE
申请人:CRYOCAPCELL
公开号:WO2019175407A1
公开(公告)日:2019-09-19
The present invention relates to the field of microscopy, preferably electron microscopy. Especially, the present invention concerns an embedding medium for imaging a biological sample by microscopy comprising: - from 60% to 99% wt. of a glycol dimethacrylate selected from alkyiene glycol dimethacrylate and/or oligo( alkyiene glycol) dimethacrylate; - from 0% to 38% wt. of a polyalkyiene glycol diacrylate or of a polyalkylene glycol methacrylate; said polyalkylene glycol diacrylate or polyalkylene glycol methacrylate being optionally substituted by at least one hydrophilic group such as hydroxyl, amino, or an oxo group; - at least one additive, preferably comprising at least one heavy metal salt or lanthanide salt; and - from 0.1% to 2% wt. of a radical polymerization initiator. The present invention also refers to the electro-conductive material resulting from the polymerization of the embedding medium of the invention, and the process and kits of preparation thereof; said material embedding at least one biological sample. The present invention also relates to a method for imaging by microscopy, a biological sample comprising using the embedding medium and/or the electro-conductive material of the invention.