butyryl-L-arginine | 160200-97-5

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: butyryl-L-arginine
• 英文别名: arginine butyrate；Nα-butyroyl-L-arginine；N-butyryl arginine；(2S)-2-(butanoylamino)-5-(diaminomethylideneamino)pentanoic acid
• CAS: 160200-97-5
• 化学式: C₁₀H₂₀N₄O₃
• 分子量: 244.294
• InChiKey: XHXFSMZBXZZZIT-ZETCQYMHSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.8
• 重原子数：
  17
• 可旋转键数：
  8
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.7
• 拓扑面积：
  131
• 氢给体数：
  4
• 氢受体数：
  4

反应信息

• 作为产物：
  描述：

  L-精氨酸 、 丁酸 在 adenosine monophosphate ligase SfaB from Streptomyces thioluteus 、 5’-三磷酸腺苷 、 magnesium chloride 、 Cleland's reagent 作用下， 以 aq. buffer 为溶剂， 反应 6.0h， 生成 butyryl-L-arginine
  参考文献：
  名称：

  重新利用3-异氰基丁酸腺苷酸化酶SfaB进行多功能酰胺化和硫酯化

  摘要：

  微生物天然产物的基因组挖掘使化学家不仅能够发现具有新颖骨架的生物活性分子，而且能够识别催化多种化学反应的酶。探索这些生物合成酶的底物混杂性和催化机理有助于开发潜在的生物催化剂。SfaB是一种形成酰基腺苷酸的酶，可在由硫链霉菌产生的二异腈自然产物SF2768的生物合成途径中，对独特的结构单元3-异氰基丁酸进行腺苷酸化。，并且该AMP连接酶被证明可以接受各种短链脂肪酸（SCFA）。在本文中，我们将SfaB重新用于催化那些SCFA与各种胺或硫醇亲核试剂之间的酰胺化或硫酯化反应，从而提供了另一种酶促方法来体外制备相应的酰胺和硫酯。

  DOI：

  10.1002/anie.202010042

文献信息

• Method for inactivating viruses by addition of slightly acidic arginine
  申请人：Ajinomoto Co., Inc.

  公开号：EP1958626A1

  公开（公告）日：2008-08-20

  The present invention provides a method for conveniently producing a protein formulation with viruses being inactivated, without impairing the quality of the obtained protein formulation, characterized by including the step of exposing the protein formulation contaminated with the viruses to a 0.1-2M aqueous solution of arginine, an arginine derivative or a mixture thereof, the aqueous solution being adjusted to pH 3.5 to 5. The present invention also provides a virus inactivation method characterized by including the step of bringing a virus-containing object into contact with a 0.1-2M aqueous solution of arginine, a arginine derivative or a mixture thereof, the aqueous solution being adjusted to pH 3.5 to 5.

  本发明提供了一种方便地生产灭活病毒的蛋白质制剂的方法，而不会损害所获得的蛋白质制剂的质量，其特征在于包括将被病毒污染的蛋白质制剂暴露于0.1-2M的精氨酸、精氨酸衍生物或其混合物的水溶液中，水溶液的pH值调节至3.5至5。本发明还提供了一种病毒灭活方法，其特征在于包括使含有病毒的物体与 0.1-2M 的精氨酸、精氨酸衍生物或其混合物水溶液接触的步骤，该水溶液的 pH 值被调节至 3.5 至 5。
• PROTEIN REFORMING METHOD
  申请人：Ajinomoto Co., Inc.

  公开号：EP2281827A1

  公开（公告）日：2011-02-09

  The present invention provides a method for producing a protein which has restored a higher-order structure of a native state thereof: by bringing the protein which has lost a higher-order structure thereof into contact at pH 6.5 to 9.0 to a 1 to 3% aqueous solution of a specific surfactant, such as lauroylglutamic acid to obtain a solubilized solution of the protein; and adding the obtained solubilized solution to a buffer with pH 6.5 to 9.0 containing arginine or an arginine derivative at a level of 0.1 to 1.2 M to lower the concentration of the specific surfactant, such as lauroylglutamic acid, in an obtained mixture solution down to 0.02 to 0.275%. According to the present invention, it is possible to easily restore a higher-order structure of a native state of a protein while smoothly detaching a surfactant from the protein.

  本发明提供了一种生产恢复了原生态高阶结构的蛋白质的方法：将失去高阶结构的蛋白质在pH6.5至9.0的特定表面活性剂（如月桂酰谷氨酸）的1%至3%的水溶液接触，以获得该蛋白质的增溶溶液；并将所获得的增溶溶液加入pH值为6.5至9.0的缓冲液中，该缓冲液中含有0.1至1.2M的精氨酸或精氨酸衍生物，以将所获得的混合物溶液中的特定表面活性剂（如月桂酰谷氨酸）的浓度降至0.02%至0.275%。根据本发明，可以在使表面活性剂与蛋白质顺利分离的同时，轻松恢复蛋白质原生状态的高阶结构。
• METHOD FOR PREPARING POLYMERIC PROTEIN COMPOSED OF MONOMERIC PROTEIN PRODUCED BY FUSING PROTEIN HAVING IMMUNOGLOBULIN FOLD STRUCTURE TO PROTEIN CAPABLE OF SERVING AS SUBUNIT STRUCTURE
  申请人：Ajinomoto Co., Inc.

  公开号：EP2725104A1

  公开（公告）日：2014-04-30

  The present invention provides a method for producing a multimeric protein consisting of a monomeric protein obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of: (A) preparing the monomeric protein having an insoluble granular form in cells of a microorganism; (B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution containing lauroyl-L-Glu; (C) diluting a solution obtained in step (B) in a buffer containing arginine hydrochloride to lower a concentration of lauroyl-L-Glu; and (D) replacing a solvent of a solution obtained in step (C) with a buffer using gel filtration chromatography or the like.

  本发明提供了一种生产多聚体蛋白质的方法，该多聚体蛋白质由具有免疫球蛋白折叠结构的蛋白质与可作为亚单位结构的蛋白质融合得到的单体蛋白质组成，该方法包括以下步骤： (A) 在微生物细胞中制备具有不溶性颗粒状的单体蛋白； (B) 用含有月桂酰-L-Glu 的水溶液增溶步骤(A)中制备的单体蛋白； (C) 在含有盐酸精氨酸的缓冲液中稀释步骤(B)中得到的溶液，以降低月桂酰-L-谷氨酰的浓度；以及 (D) 使用凝胶过滤色谱法或类似方法将步骤(C)中得到的溶液的溶剂替换为缓冲液。
• METHOD FOR AFFINITY CHROMATOGRAPHY
  申请人：Novartis AG

  公开号：EP3272764A1

  公开（公告）日：2018-01-24

  The invention provides a washing method for affinity chromatography in which a wash solution comprising arginine, or an arginine derivative, and a nonbuffering salt, preferably at high pH, greater than 8.0, is effective in removing impurities, such as high molecular weight species and host cell proteins, while also increasing product concentration in the eluate and maintaining a high percent yield of recovered product.

  本发明提供了一种用于亲和层析的洗涤方法，在这种方法中，由精氨酸或精氨酸衍生物和非缓冲盐组成的洗涤溶液，最好在高 pH 值（大于 8.0）条件下，能有效去除杂质，如高分子量物质和宿主细胞蛋白，同时还能提高洗脱液中的产物浓度，并保持较高的回收产物产率。
• METHOD FOR PRODUCING REFOLDED PROTEIN BY USING FLOW MICROREACTOR, AND PROTEIN REFOLDING APPARATUS
  申请人：AJINOMOTO CO., INC.

  公开号：EP3878856A1

  公开（公告）日：2021-09-15

  The present invention provides a method for producing a refolded protein, the method comprising a step for mixing, inside a micro mixer, a buffer and a solubilization solution for a protein that has lost higher order structure or that has become insoluble.

  本发明提供了一种生产再折叠蛋白质的方法，该方法包括在微型混合器内混合缓冲液和增溶液的步骤，缓冲液用于已失去高阶结构或变得不溶解的蛋白质。