申请人:——
公开号:US20020111500A1
公开(公告)日:2002-08-15
Biocatalysis is used to prepare 4-hydroxy-3[2H]-furanones from substituted benzenes. A substituted benzene is enzymatically oxidized to form a diol-diene compound, which is then oxidized and cyclized to form a 4-hydroxy-3[2H]-furanone. Dioxygenases are used to perform the enzymatic oxidation. In addition, methods of obtaining improved dioxygenases are provided. Compositions including one or more of the intermediate compounds in the biocatalysis method, the resulting 4-hydroxy-3[2H]-furanone compounds, and improved enzymes are also provided.
生物催化用于从取代苯制备 4-羟基-3[2H]-呋喃酮。取代苯经酶促氧化形成二醇二烯化合物,再经氧化和环化形成 4-羟基-3[2H]-呋喃酮。二氧 化酶用于进行酶促氧化。此外,还提供了获得改进的二氧化酶的方法。还提供了包括生物催化方法中的一种或多种中间化合物、所得的4-羟基-3[2H]-呋喃酮化合物和改良酶的组合物。