申请人:MELOY LABORATORIES, INC.
公开号:EP0137691A1
公开(公告)日:1985-04-17
Process for the production of IFN-γ comprising incubating for a period of time at least sufficient for the production of IFN-y, a viable cell suspension which includes (a) leukocytes, (b) an inducer for production of IFN-γ, and (c) a modulator for said inducer selected from the group consisting of mezerein and 12, 13-phorbol butyrate, said inducer and said modulator being present in said suspension in amount sufficient to stimulate production of IFN-y; process for producing substantially homogeneous IFN-y; process for producing IFN-γ mRNA; and cloning process for production of IFN-γ. Products disclosed are substantially homogeneous IFN-γ and its 2 species; and the host cells containing the recombinant cloning vehicle containing DNA segment coding for IFN-γ.
生产 IFN-γ 的工艺,包括将一种有活力的细胞悬浮液培养一段时间,至少足以产生 IFN-y,该悬浮液包括(a)白细胞,(b)生产 IFN-γ 的诱导剂,和(c)选自甲氧苄啶和 12,13-薄荷醇丁酸酯的组中的所述诱导剂的调节剂,所述诱导剂和所述调节剂在所述悬浮液中的含量足以刺激 IFN-y 的产生;生产基本均一的 IFN-y 的工艺;生产 IFN-γ mRNA 的工艺;以及生产 IFN-γ 的克隆工艺。公开的产品有基本均一的 IFN-γ 及其 2 种;以及含有重组克隆载体的宿主细胞,该载体含有编码 IFN-γ 的 DNA 片段。