3-O-β-D-galactopyranosyl-D-xylose | 14087-18-4

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: 3-O-β-D-galactopyranosyl-D-xylose
• 英文别名: Gal(b1-3)aldehydo-Xyl；(2R,3S,4R)-2,4,5-trihydroxy-3-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxypentanal
• CAS: 14087-18-4
• 化学式: C₁₁H₂₀O₁₀
• 分子量: 312.274
• InChiKey: ZTTRCZJSZGZSTB-GDIDZOIGSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -4.4
• 重原子数：
  21
• 可旋转键数：
  7
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.91
• 拓扑面积：
  177
• 氢给体数：
  7
• 氢受体数：
  10

文献信息

• Enzymatic method of producing 4-O-B-D-galactopyranosyl-D-xylose, 4-O-B-D-galactopyranosyl-D-xylose obtained using said method, compositions contain same and the use thereof in evaluating intestinal lactase
  申请人：——

  公开号：US20040241811A1

  公开（公告）日：2004-12-02

  An enzymatic process to obtain 4-O-&bgr;-D-galactopyranosyl-D-xylose useful in compositions or solutions in the in vivo evaluation of intestinal lactose activity in humans, that comprises the steps of preparing a reaction mixture of D-xylose, a &bgr;-D-galactopyranoside and a reaction medium that comprises water buffered to a pH between 5.0 and 9.0; adding 10 to 1,000 units of &bgr;-D-galactosidase per gram of &bgr;-D-galactopyranoside; subjecting the reaction mixture to a reaction or a temperature between a temperature higher than the freezing point of the reaction mixture and 45° C., for 2 to 48 hours; for the reaction by deactivation of the &bgr;-D-galactosidase; and to isolate and crystallize the fractions that contain 4-O-&bgr;-D-galactopyranosyl-D-xylose from a crystallization mixture selected between mixtures of acetone/methanol in a ratio between 5/1 to 20/1 and mixtures of acetone/water in a ratio between 5/1 to 20/1.

  一种酶法过程，用于制备4-O-β-D-半乳糖苷-D-木糖，用于人体肠内乳糖活性的组成物或溶液的体内评估，包括以下步骤：制备反应混合物，其中包括D-木糖，β-D-半乳糖苷和缓冲至pH在5.0至9.0之间的水反应介质；每克β-D-半乳糖苷添加10至1,000单位的β-D-半乳糖苷酶；将反应混合物在高于冰点的温度和45°C之间的温度下反应2至48小时；通过去活化β-D-半乳糖苷酶进行反应；并从丙酮/甲醇比例在5/1至20/1之间的结晶混合物或丙酮/水比例在5/1至20/1之间的结晶混合物中分离和结晶含有4-O-β-D-半乳糖苷-D-木糖的分数。
• USE OF O-BETA-D-GALACTOPYRANOSYL-D-XYLOSES FOR THE PREPARATION OF COMPOSITIONS AND SOLUTIONS INTENDED TO THE EVALUATION OF INTESTINAL LACTASE, AND PRODUCTION PROCESS
  申请人：CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

  公开号：EP0819764A1

  公开（公告）日：1998-01-21

  There is disclosed the use of O-β-D-galactopyranosyl-D-xyloses, particularly 2-O-β-D-galactopyranosyl-D-xylose, and 3-O-β-D-galactopyranosyl-D-xylose, for the preparation of compositions and solutions intended to the evaluation of the intestinal lactase, which present an affinity to the substantially enhanced lactase. There is also disclosed a process for producing O-β-D-galactopyranosyl-D-xyloses, comprising: reacting a D-xylose and a substrate of β-D-galactopyranoside in the presence of an enzyme β-galactosidase, in an aqueous medium buffered to a pH comprised between 5.0 and 9.0 and at temperature between 4 and 37°C; deactivating the β-galactosidase by heating at 100°C when reaching the maximum yield of formation of disaccharides detected through thin layer chromatography, and isolating the formed disaccharides through filtration in a packed column with a thinner selected between water and water/ alcohol.

  公开了O-β-D-吡喃半乳糖基-D-木糖的用途，特别是2-O-β-D-吡喃半乳糖基-D-木糖和3-O-β-D-吡喃半乳糖基-D-木糖，用于制备用于评估肠道乳糖酶的组合物和溶液，这些组合物和溶液对大大增强的乳糖酶具有亲和力。还公开了一种生产 O-β-D-吡喃半乳糖苷-D-木糖的工艺，包括：在β-半乳糖苷酶存在下，在 pH 缓冲至 5.0 至 9.当通过薄层色谱法检测到形成二糖的产量达到最大值时，通过在 100℃下加热使 β-半乳糖苷酶失活，并通过使用在水和水/醇之间选择的稀释剂的填料柱过滤分离形成的二糖。
• ENZYMATIC METHOD OF PRODUCING 4-0-B-D GALACTOPYRANOSYL-D-XYLOSE, 4-0-B-D-GALACTOPYRANOSYL-D-XYLOSE OBTAINED USING SAID METHOD, COMPOSITIONS CONTAINING SAME AND THE USE THEREOF IN EVALUATING INTESTINAL LACTASE
  申请人：CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

  公开号：EP1408118A1

  公开（公告）日：2004-04-14

  An enzymatic process to obtain 4-O-β-D-galactopyranosyl-D-xylose useful in compositions or solutions in the in vivo evaluation of intestinal lactose activity in humans, that comprises the steps of preparing a reaction mixture of D-xylose, a β-D-galactopyranoside and a reaction medium that comprises water buffered to a pH between 5.0 and 9.0; adding 10 to 1,000 units of β-D-galactosidase per gram of β-D-galactopyranoside; subjecting the reaction mixture to a reaction or a temperature between a temperature higher than the freezing point of the reaction mixture and 45°C, for 2 to 48 hours; for the reaction by deactivation of the β-D-galactosidase; and to isolate and crystallize the fractions that contain 4-O-β-D-galactopyranosyl-D-xylose from a crystallization mixture selected between mixtures of acetone/methanol in a ratio between 5/1 to 20/1 and mixtures of acetone/water in a ratio between 5/1 to 20/1.

  一种获得 4-O-β-D-吡喃半乳糖基-D-木糖的酶法工艺，该工艺可用于对人体肠道乳糖活性进行体内评估的组合物或溶液中，该工艺包括以下步骤：制备由 D-木糖、β-D-吡喃半乳糖苷和反应介质组成的反应混合物，反应介质包括 pH 值在 5.0 和 9.0 之间的缓冲水；每克 β-D 吡喃半乳糖苷中加入 10 至 1 000 个单位的 β-D 半乳糖糖苷酶；将反应混合物置于温度高于或等于 β-D 吡喃半乳糖苷的温度的条件下。0；每克β-D-吡喃半乳糖苷中加入10至1,000单位的β-D-半乳糖苷酶；将反应混合物置于高于反应混合物冰点的温度和45℃之间的温度下反应或加热2至48小时；通过使β-D-半乳糖苷酶失活来进行反应；从丙酮/甲醇混合物（比例在 5/1 至 20/1 之间）和丙酮/水混合物（比例在 5/1 至 20/1 之间）中选择的结晶混合物中分离并结晶出含有 4-O-β-D 吡喃半乳糖基-D-木糖的馏分。
• USE OF 4-GALACTOSYL-XYLOSE IN HUMANS FOR THE IN VIVO EVALUATION OF INTESTINAL LACTASE AS A NON-INVASIVE DIAGNOSTIC TEST OF THE DEFICIENCY OF SAID ENZYME
  申请人：CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

  公开号：EP1553188A1

  公开（公告）日：2005-07-13

  The invention relates to the use in humans of a method which is based on the use of 4-galactosyl-xylose and which involves an alternative technique to evaluate xylose in urine. The method consists of measuring the concentration of xylose in the blood following the oral administration of 4-galactosyl-xylose to the patient, the use of said method being even more suitable in the case of newborns. The invention also relates to the technology used for the in vivo evaluation of intestinal lactase activity, by way of a non-invasive test for the diagnosis of a deficiency of said enzyme, which consists of directly evaluating the overall activity of the enzyme in the individual and not in assessing the consequences of said deficiency. The inventive method does not require specialized equipment, does not cause any obvious discomfort to patients with a lactase deficiency and is extremely reliable, thereby eliminating the inconveniences of currently used diagnostic tests.

  本发明涉及一种以 4-半乳糖基木糖为基础的方法在人体中的应用，该方法涉及一种评估尿液中木糖含量的替代技术。该方法包括在患者口服 4-半乳糖基木糖后测量血液中木糖的浓度，该方法更适用于新生儿。本发明还涉及用于体内评估肠道乳糖酶活性的技术，通过无创检测诊断所述酶的缺乏，包括直接评估个人体内酶的整体活性，而不是评估所述缺乏的后果。本发明的方法不需要专门的设备，不会对乳糖酶缺乏症患者造成任何明显的不适，而且非常可靠，从而消除了目前使用的诊断测试所带来的不便。
• Use of 4-galactosyl-xylose in humans for the in vivo evaluation of intestinal lactase as a non-invasive diagnostic test of the deficiency of said enzyme
  申请人：Aragon Reyes Jose Juan

  公开号：US20050238580A1

  公开（公告）日：2005-10-27

  The invention relates to the use in humans of a method which is based on the use of 4-galactosyl-xylose and which involves an alternative technique to evaluate xylose in urine. The method consists of measuring the concentration of xylose in the blood following the oral administration of 4-galactosyl-xylose to the patient, the use of said method being even more suitable in the case of newborns. The invention also relates to the technology used for the in vivo evaluation of intestinal lactase activity, by way of a non-invasive test for the diagnosis of a deficiency of said enzyme, which consists of directly evaluating the overall activity of the enzyme in the individual and not in assessing the consequences of said deficiency. The inventive method does not require specialized equipment, does not cause any obvious discomfort to patients with a lactase deficiency and is extremely reliable, thereby eliminating the inconveniences of currently used diagnostic tests.

  本发明涉及一种以 4-半乳糖基木糖为基础的方法在人体中的应用，该方法涉及一种评估尿液中木糖含量的替代技术。该方法包括在患者口服 4-半乳糖基木糖后测量血液中木糖的浓度，该方法更适用于新生儿。本发明还涉及用于体内评估肠道乳糖酶活性的技术，通过无创检测诊断所述酶的缺乏，包括直接评估个人体内酶的整体活性，而不是评估所述缺乏的后果。本发明的方法不需要专门的设备，不会对乳糖酶缺乏症患者造成任何明显的不适，而且非常可靠，从而消除了目前使用的诊断测试所带来的不便。